Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid. One of the most frequent problems that I help troubleshoot involves viral vectors and plasmids with repeating sequences. Repeating sequences within a plasmid can undergo ...
New to the lab? Or looking for ways to supplement your teaching? You’re in luck! Addgene’s Intro to the Lab Bench series was born from the shift to remote learning due to COVID-19. We had many protocols for plasmid cloning and working with viral vectors, but what we lacked were ...
Antibodies are used in many different experiments that require scientists to detect proteins in their samples. One technique that relies heavily on antibodies is ELISA, which stands for Enzyme-linked Immunosorbent Assay. ELISA are used to detect proteins within a 96- or ...
We’re making some moves over here at Addgene...and they’re looping?
What is polymerase chain reaction? If you have ever worked in a molecular biology laboratory you have likely done a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied many many times in a short time period. PCR was invented in ...
We are excited to announce the recipients of this year’s Michael Davidson and Roger Tsien Commemorative Conference Awards. Congratulations to the five winners!
We’re happy to introduce the newest member on Addgene’s Board of Directors, Bart Newland. Newland is General Counsel at Atalanta, an early-stage biotech company that is developing RNAi therapeutics to treat neurodegenerative diseases. He has earned a B.S. in microbiology and ...
