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27 Hot Plasmids from 2016

Posted by Tyler Ford on Dec 22, 2016 10:03:47 AM

Every quarter we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles highlight the main features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it easier for you to find and use the plasmids you need. You can find all the hot plasmids from 2016 below. With over 45,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but be sure to let us know if you'd like to write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the Addgene Podcast.

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Topics: Hot Plasmids, News

Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

Posted by Tyler Ford on Dec 20, 2016 10:30:00 AM

In this episode of the Addgene Podcast, we sit down with Kwabena Duedu, a researcher at the University of Health and Allied Sciences in Ghana. He’s done research at a number of institutions in Ghana and most recently got his PhD in cell and molecular biology at the University of Edinburgh where he worked on developing novel systems for bioconversion of cellulosic biomass to useful products under Professor Christopher E. French.


Dr. Duedu was recently awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant. For this grant, we’re working with fellow nonprofit, Seeding Labs, to distribute plasmids to researchers in developing regions and thereby accelerate their research. Kwabena came across the plasmid grant in Seeding Lab’s Newsletter and recently visited Cambridge to spend some time learning and doing research at Novartis.

Listen to learn how Kwabena plans to use his research experiences as well as plasmids from Addgene to bring new opportunities to Ghanaian scientists

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Topics: Interview, Synthetic Biology, Podcast

Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Posted by Beth Kenkel on Dec 15, 2016 10:57:02 AM

Restriction enzyme cloning is the workhorse of molecular cloning; however, one of its biggest limitations is that sequence modifications can only be made at restriction enzyme cut sites. The lambda red system is an alternative method that can be used for cloning or genome engineering and is based on homologous recombination. It allows for direct modification of DNA within E. coli and is independent of restriction sites. The lambda red system is derived from the lambda red bacteriophage and its use as a genetic engineering tool is frequently called recombineering - short for homologous recombination-mediated genetic engineering.  It can be used to make an assortment of modifications: insertion and deletion of selectable and non-selectable sequences, point mutations or other small base pair changes, and the addition of protein tags. It also has the flexibility to modify the E. coli chromosome, plasmid DNA or BAC DNA. 

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Topics: Genome Engineering, Techniques, Microbiology

Mesothelioma - Causes, Symptoms, and Treatment

Posted by Guest Blogger on Dec 13, 2016 10:30:00 AM

This post was contributed by the Mesothelioma Cancer Alliance.

We have learned much about the causes of cancer and the different avenues that can be used to treat it. For those who are running out of hope with more traditional treatments, such as surgery, radiation, and chemotherapy, immunotherapy is coming to the fore as a cutting edge form of cancer treatment. With the goal of the Cancer Moonshot Initiative being to cure cancer, more funding and research opportunities are being provided to immunotherapy than ever before. Although different types of cancer have different challenges and obstacles to overcome, mesothelioma sufferers can see great promise in up and coming treatments like immunotherapy.

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Topics: Cancer

Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

Posted by Michael G. Lemieux on Dec 8, 2016 10:30:00 AM

If you’re into cloning, you’re probably aware that there are several methodologies currently available for approaching it. These include the traditional restriction enzyme/ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as well as several others. Each method is unique and relies on specific components that are key to the cloning reaction. Understanding the specific components is essential for choosing the correct cloning method for your own experiments, and here we will focus on a unique gene that makes the popular GatewayTM method possible: ccdB. But what is ccdB, what role does it play in modern cloning, and why should you learn more about it? Read on to find out how ccdB can make your cloning experiments a little easier.

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Topics: Plasmids 101, Plasmid Cloning

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