Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Immunofluorescence (IF), is an immunoassay that brings to light the cellular world. The technique allows you to ask questions like: “Where does my protein of interest live within a cell,” “Does this disease change the architecture of my cells,” or “How does this mutation impact ...
Many neuroscience experiments that require gene expression in a specific cell type rely on transgenic models that express recombinases like Cre or Flp in their cells of interest and recombinase-dependent AAV vectors for selective transgene expression. While this is a powerful ...
There are a lot of ways to classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you heard of an antibody isotype? An isotype determines several key characteristics of an antibody as well as the role it plays in an immune response. But ...
This post was contributed by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College of Medicine. Several sets of near-infrared fluorescent proteins (NIR FPs) and biosensors have been created recently. As developers of many of these probes, we decided to ...
Every few months we highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need.
Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid. One of the most frequent problems that I help troubleshoot involves viral vectors and plasmids with repeating sequences. Repeating sequences within a plasmid can undergo ...
New to the lab? Or looking for ways to supplement your teaching? You’re in luck! Addgene’s Intro to the Lab Bench series was born from the shift to remote learning due to COVID-19. We had many protocols for plasmid cloning and working with viral vectors, but what we lacked were ...
