Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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If you’ve been following our antibody blog posts, then you likely already have a good idea of the basics of immunoimaging. A scientist conjugates an antibody with a signaling molecule, the antibody binds to a protein, and then voila! Wherever your protein of interest is, a ...
This post was contributed by guest blogger Daniel M. Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase DNMT3A allows ...
Immunoprecipitation (IP) uses immobilized antibodies, or immunoglobulins, to isolate a specific protein out of a complex mix. Using this technique, users can look for the presence or absence of a protein, determine if a protein is up or downregulated, examine a protein’s ...
It’s finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing temperatures, our first of the season snow flurries, dusting off the winter coats and hats, and our annual Deck The Lab holiday decorating contest!
A 2017 survey found that many researchers feel they lack formal training in a variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the lab and by offering advice on ...
It’s time for another edition of “What’s New in CRISPR” - where we highlight a few of the newest CRISPR plasmids available at Addgene. If you want to read more content related to CRISPR technology, subscribe to our CRISPR blog content. In this post we will cover: Miniature ...
Immunofluorescence (IF), is an immunoassay that brings to light the cellular world. The technique allows you to ask questions like: “Where does my protein of interest live within a cell,” “Does this disease change the architecture of my cells,” or “How does this mutation impact ...
