Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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If you work in a lab that regularly does immunoimaging, there’s likely a large collection of antibodies in your lab. Perhaps you’re even in that mythical place where antibodies are well-organized, documented, and easy to find (though we might need to see it to believe it.) As ...
I first realized I might have some minor skills in conflict resolution after college, when I discovered that many of my old suitemates stopped talking to each other if I was not there to arbitrate for them. They were different people with some very different opinions, and some ...
One of our favorite holiday traditions is our annual Twelve Days of CRISPR campaign! But if you missed our tweets featuring the best of CRISPR in 2021 and useful resources, fear not! You can find all of them here.
If you’ve been following our antibody blog posts, then you likely already have a good idea of the basics of immunoimaging. A scientist conjugates an antibody with a signaling molecule, the antibody binds to a protein, and then voila! Wherever your protein of interest is, a ...
This post was contributed by guest blogger Daniel M. Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase DNMT3A allows ...
Immunoprecipitation (IP) uses immobilized antibodies, or immunoglobulins, to isolate a specific protein out of a complex mix. Using this technique, users can look for the presence or absence of a protein, determine if a protein is up or downregulated, examine a protein’s ...
It’s finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing temperatures, our first of the season snow flurries, dusting off the winter coats and hats, and our annual Deck The Lab holiday decorating contest!
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