Rachel is a Science Communications Specialist at Addgene. She manages the blog and social media.
If you’ve ever used Golden Gate Assembly for cloning, you might be familiar with the rules of thumb for designing your overhang sets. But are those rules the best way to design GGA overhang sets, particularly for high-complexity reactions?
If you study proteins, you’re probably quite interested in the canonical 20 amino acids. But in your quest to learn more about your protein of interest, you may find the available amino acids a bit…limiting. It may be time, then, to look towards genetic code expansion (GCE): ...
Addgene is excited to announce the launch of our partnership with the Institute for Protein Innovation (IPI). Together, we’ll be working to provide researchers with a collection of IPI’s well-validated and ready-to-use recombinant antibodies. The first collection, a set of ...
IBEX is a highly multiplexed tissue imaging method that uses iterative staining and chemical bleaching to allow for high-resolution imaging without degrading the tissue (Radtke et al., 2020, 2022). I do mean highly multiplexed: you can image over 65 different parameters using ...
Last summer, we posted about our bioinformatics quest, which led us to Seqera Labs, a company that provides open source workflow orchestration software for data pipeline processing (Nextflow), cloud infrastructure, and secure collaboration.
When it comes to using antibodies in the lab, we focus on a lot on the variable domain and not so much on the constant, or Fc, domain. Sure, we all know that the Fc domain provides structure, determines isotypes, and provides a place for secondary antibodies to bind. We also ...
Public speaking is not just one of the most common phobias–it’s a key part of being a scientist and an incredibly useful transferable skill. Being able to stand up in front of an audience and convey your ideas clearly and strategically is powerful in almost any career. It allows ...
Running a Western blot can be a bit daunting for first-timers. That's why we gave our written protocol a video companion! Follow along as Addgenie Meghan and Abi run a Western blot and share tips for this tricky application.
