Addgenie Mary Gearing contributed to the content of this article.
Biosensors (‘biological sensors’) are biological tools that monitor a process or detect a given molecule. The sensor component is usually a protein that undergoes a conformational change in response to the molecule it detects. This change then generates a reporter signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent reporters being especially popular. We’ll give you an introduction to fluorescent biosensors, but keep in mind that there is a lot of variety in how biosensors work, and you should always check the associated publication for the specifics of your chosen plasmid.