This post was contributed by guest blogger, Krissy Lyon, a PhD candidate in Neuroscience at Harvard University. Just as computers, cell phones, and cars become more technologically advanced leaving earlier versions obsolete, the techniques we use in lab are replaced by improved ...
This blog post was contributed by guest blogger Kate Palozola Traditional lab notebooks just won't cut it for bioinformatics. All kinds of biologists are finding themselves using computational approaches to analyze large data sets (myself included) and we are faced with finding ...
Addgene is proud to announce that we recently acquired the ability to distribute plasmids with the piggyBac™ transposon. These plasmids, when combined with a source of piggyBac™ transposase (available from a licensed distributor) allow you to quickly transfer a DNA sequence from ...
This post was contributed by ATCC Outreach Scientist Nick Amiss. Cell culture in the present day Cell culture is a widespread tool used in the fields of oncology, virology, immunology, microbiology, and pharmacology (many of which are represented in Addgene's Special ...
This post was contributed by guest blogger Erik L. Snapp. Stop using EGFP/GFP for fusion proteins! Despite multiple studies in high profile journal articles, many researchers remain unaware that EGFP/GFP is prone to forming noncovalent dimers. This property of EGFP can lead to ...
This post was contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela Silver. Most types of biological motion (whether endocytosis, vesicle trafficking, or muscle contractions) are produced by orchestrated movements of networks of proteins ...
This post was contributed by guest blogger Alan Wong. The complexity of biological systems can hinder our attempts to study and engineer them, but what if we had a simple tool that allowed us to rapidly decode the complexity? The CombiGEM-CRISPR technology was developed with the ...
This post was contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’s lab. CRISPR-Cas9 (Cas9) is an RNA-guided nuclease that targets and cuts genomic DNA. The interplay between Cas9 (which causes the breaks) and host cell DNA repair factors ...