How many times have you looked at a diagram depicting transcription, or DNA repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the context of chromatin?” Though it’s true that adding histones and chromatin architecture to ...
The central dogma in molecular biology is DNA→RNA→Protein. To synthesize a particular protein DNA must first be transcribed into messenger RNA (mRNA). mRNA can then be translated at the ribosome into polypeptide chains that make up the primary structure of proteins. Most ...
This post was contributed by guest blogger Clare O'Connor an Associate Professor at Boston College. National reports stress the importance of providing authentic research experiences to undergraduate students (1, 2), but educators face significant challenges in designing ...
Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like ...
One way to define a protein’s purpose is by its protein-protein interactions (PPIs). These interactions are often modeled as binary relationships, i.e. protein A interacts with protein B; but proteins are social biomolecules. They can be part of multiple dynamic and overlapping ...
Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. Below you'll find ...
Antibodies are a go-to tool for detecting a protein of interest in cells and tissues. Although antibody production is well established, it’s also a process that’s difficult for individual labs to complete. The nanobody based RANbody platform from the Sanes Lab overcomes this ...
This post was contributed by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs. When do you need a high fidelity ligase—and when is an alternative ligase a better choice? And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes ...
