By Beth Kenkel
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This post was contributed by guest blogger Matthew J. Niederhuber, a graduate student at UNC Chapel Hill. Chromatin immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method for mapping where a protein binds genome-wide, and has been widely ...
We’ve talked a lot about the quality control process at Addgene by introducing our new sequencing partner seqWell and going into detail about how we use next generation sequencing results to perform quality control on deposited plasmids. We’ve also talked about how our new ...
This post was contributed by guest blogger Jon Backstrom, a biochemist in the Vanderbilt Eye Institute and Tonia Rex's lab. A common strategy to determine the binding kinetics of a purified protein involves immobilization on a solid support. This allows washing away of unbound ...
Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region drives transcription of a target gene, it therefore determines the timing of gene expression and largely defines the amount of recombinant protein that will be produced. Many ...
This post was contribued by guest bloggers Aline and Benjamin Glick from SnapGene. SnapGene was created to meet a need. While there were software tools available to biomedical researchers manipulating DNA sequences on a daily basis, many found these tools inadequate for ...
This post was contributed by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital. A little over a decade back when Yamanaka and colleagues reported that it is possible to reprogram differentiated cells into induced pluripotent stem cells (iPSCs) by the ...
In the fourth episode of our Hot Plasmids podcast series, you'll learn about new libraries for studying B. subtilis biology, plasmids for enhancing iPSC production, and CRISPR tools for plants. You can find additional hot plasmids in our quarterly newsletter or on our hot ...
