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This post was contributed by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital. A little over a decade back when Yamanaka and colleagues reported that it is possible to reprogram differentiated cells into induced pluripotent stem cells (iPSCs) by the ...
In the fourth episode of our Hot Plasmids podcast series, you'll learn about new libraries for studying B. subtilis biology, plasmids for enhancing iPSC production, and CRISPR tools for plants. You can find additional hot plasmids in our quarterly newsletter or on our hot ...
In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds, thousands, or even a million plasmids. These libraries are some of Addgene’s most exciting and versatile offerings! We recently re-amplified our distribution stock of the ...
In the third episode of our Hot Plasmids podcast series, you'll learn about optogenetics tools for controlling protein activity, methods for increasing CRISPR editing efficiency, new CRISPR base editors, DIY DNA ladders. You can find additional hot plasmids in our newsletter or ...
This post was contributed by guest blogger Katrin Michel. Cre-lox is an incredibly popular and powerful site specific recombinase (SSR) system, but it only gives you a single level of control without modification - either Cre is there or it’s not. Cre-mediated possibilities for ...
This post was contributed by guest blogger Iris Lindberg, Professor at the University of Maryland School of Medicine. In the Lindberg Lab we often make cell lines that overexpress genes of interest; more recently we have also been using Addgene CRISPR vectors to generate cell ...
This post was contributed by guest blogger Lydia Morrison from New England Biolabs. What is DNA assembly? In the context of cloning, DNA assembly refers to a method of physically joining multiple fragments of DNA to create a synthetically designed DNA sequence. There are ...
