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pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Posted by Guest Blogger on Dec 14, 2017 9:08:09 AM

This post was contributed by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital.

A little over a decade back when Yamanaka and colleagues reported that it is possible to reprogram differentiated cells into induced pluripotent stem cells (iPSCs) by the addition of reprogramming (or “Yamanaka”) factors, they changed the landscape of regenerative medicine. Their work opened up vast possibilities for the clinical and therapeutic applications of iPSCs. The generation of human iPSCs (hiPSCs) now provides an opportunity to develop and use patient-specific somatic cells that are otherwise difficult to obtain. These can then be used to perform cell therapy and to model diseases in vitro.

Find stem cell plasmids at Addgene

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Topics: Plasmid Technology, Hot Plasmids, Stem Cells

Hot Plasmids Episode 4: B. subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!

Posted by Tyler Ford on Dec 11, 2017 9:02:49 AM

In the fourth episode of our Hot Plasmids podcast series, you'll learn about new libraries for studying B. subtilis biology, plasmids for enhancing iPSC production, and CRISPR tools for plants. You can find additional hot plasmids in our quarterly newsletter or on our hot plasmids webpage.

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Topics: Hot Plasmids, Optogenetics, CRISPR, Podcast

Hot Plasmids Podcast Episode 3: Optogenetics Tools, Improved CRISPR Base Editors, & More!

Posted by Tyler Ford on Oct 25, 2017 9:00:00 AM

In the third episode of our Hot Plasmids podcast series, you'll learn about optogenetics tools for controlling protein activity, methods for increasing CRISPR editing efficiency, new CRISPR base editors, DIY DNA ladders. You can find additional hot plasmids in our quarterly newsletter or on our hot plasmids webpage.

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Topics: Hot Plasmids, Optogenetics, CRISPR, Podcast

In Memoriam - The Origins of pBABE and Generations of Cancer Research

Posted by Guest Blogger on Sep 29, 2017 12:44:40 PM

This post was contributed by guest blogger, Jay P. Morgenstern,  Ph.D., Director, Genetic Engineering & Molecular Biology at Warp Drive Bio. The post is in memoriam of his late father and pBABE’s namesake, Harold (Babe) Morgenstern.

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Topics: Hot Plasmids, Cancer

AAVs CREATed for Gene Delivery to the CNS and PNS

Posted by Beth Kenkel on Sep 28, 2017 10:01:35 AM

Adeno-associated viral (AAV) vectors are the most frequently used gene-transfer tools in the study of the brain and spinal cord, which together are known as the central nervous system (CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate, 2) they have long-term expression; and 3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience research is the lack of a method for genetically manipulating neurons throughout the whole brain. Neurons of the peripheral nervous system (PNS), which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene delivery, especially for the study of pain. While many new capsids (i.e. the part of the virus that determines tropism) have been developed that increase transduction efficiency, none allow for simple and efficient transduction of both the CNS and PNS.That is until the Gradinaru Lab at Caltech stepped up to the challenge.

Find the PHP Plasmids Here!

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Topics: Plasmid Technology, Hot Plasmids, Viral Vectors

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