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Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been difficult to study without biosensors to directly visualize their activity. In 2019, the first generation of norepinephrine sensors was developed, named GRABNE, which helped ...
Here at Addgene’s headquarters, the skies are getting dark and wintry with the end of year approaching. We’re always looking for something to brighten our day, whether it’s memories of summer or just a bright new plasmid. One area with lots of bright new plasmids is our ...
Many, many techniques are available to assess protein-protein interactions. One popular approach is to fuse a protein of interest to each part of a split fluorescent protein (FP) and measure the signal produced when the candidate proteins’ interaction brings the pieces of the FP ...
This post was contributed by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College of Medicine. Several sets of near-infrared fluorescent proteins (NIR FPs) and biosensors have been created recently. As developers of many of these probes, we decided to ...
We are excited to announce the recipients of this year’s Michael Davidson and Roger Tsien Commemorative Conference Awards. Congratulations to the five winners!
Have you ever wanted to measure expression of your protein of interest in a single cell? Or perhaps, you need to analyze a specific subset of cells in a complex population. Have you spent hours in the biosafety cabinet with cloning rings or following labor-intensive limiting ...
Streaking for single colonies is an integral part of any bacteriologist’s skill set. So when Dave Westenberg taught this concept in his microbiology lab course, he decided to add a bit of fun. He mixed together 10 E. coli strains producing different pigments, and tasked the ...