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New Neuroscience Tool: The SF-iGluSnFr Glutamate Sensor

Posted by Tyler Ford on Mar 8, 2018 9:00:00 AM

In a previous blog post we discussed how fluorescent proteins can be used to construct biosensors, biological tools that monitor processes or detect molecules. Here we’ll be diving into the details surrounding SF-iGluSnFr, a recently upgraded biosensor designed to detect glutamate.

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Topics: Fluorescent Proteins, Hot Plasmids

Technologies Enabled by NanoLuc® Luciferase

Posted by Guest Blogger on Feb 8, 2018 7:17:02 AM

This post was contributed by Kyle Hooper at Promega.

Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the lab, if you read a paper and saw an interesting construct you wished to use, you could either make it yourself or you could “clone by phone”. One of my professors was excellent at phone cloning with labs around the world and had specific strategies and tactics for getting the plasmids he wanted. Addgene makes it so much easier to share your constructs from lab to lab. Promega supports the Addgene mission statement: Accelerate research and discovery by improving access to useful research materials and information. Many of our technology platforms like HaloTag® Fusion Protein, codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from the repository. We encourage people to go to Addgene to get new innovative tools. Afterall, isn’t science better when we share?

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Topics: Fluorescent Proteins

Light Up Your Experiments with the Michael Davidson Collection

Posted by Lianna Swanson on Oct 31, 2017 9:22:25 AM

Michael Davidson (1950-2015) dedicated his scientific career to 3 major avenues – mentoring young students and instilling a strong work ethic in them, developing educational resources for microscopy, and building new fluorescent protein tools for the scientific community. Davidson took the fluorescent proteins originally developed by Roger Tsien, a frequent collaborator, and expanded on then to revolutionize the study of cell biology. In 2014, Mike Davidson deposited his plasmid tools with Addgene.

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Topics: Fluorescent Proteins

Fluorescent Tagging of Endogenous Genes with SapTrap

Posted by Michelle Cronin on Oct 12, 2017 10:26:13 AM

Since the discovery of GFP over 50 years ago, the growing spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization and function of cellular systems. FPs have been used to track protein localization, cell structure, intracellular trafficking, and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles, scientists have been able to study many cellular processes, including mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled discovery of many cellular mechanisms, there are some limitations to working with FPs. Overexpression of fluorescently tagged proteins can lead to improper protein localization, protein aggregation, or disruption of normal protein function, and ultimately misinterpretation of the protein’s cellular role.

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Topics: Fluorescent Proteins, CRISPR

Fluorescence Microscopy Techniques - Which is Best for Me?

Posted by Guest Blogger on Oct 10, 2017 9:57:00 AM

This post was contributed by Doug Richardson, Director of the Harvard Center for Biological Imaging and a Lecturer on Molecular and Cellular Biology at Harvard University.

No matter whether you are a sports photographer at the Super Bowl, a medical technologist taking an x-ray, or a biologist imaging the smallest structures of life; the key to a great image is contrast. The human visual system relies primarily on contrast to identify individual objects and perceive the world around us. Without contrast, objects simply vanish into noise.

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Topics: Fluorescent Proteins

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