By Jennifer Tsang
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Have you ever wanted to measure expression of your protein of interest in a single cell? Or perhaps, you need to analyze a specific subset of cells in a complex population. Have you spent hours in the biosafety cabinet with cloning rings or following labor-intensive limiting ...
Streaking for single colonies is an integral part of any bacteriologist’s skill set. So when Dave Westenberg taught this concept in his microbiology lab course, he decided to add a bit of fun. He mixed together 10 E. coli strains producing different pigments, and tasked the ...
To respond to environmental conditions, cells must be able to detect extracellular stimuli. One way they do so is through G protein-coupled receptors (GPCRs), receptors that play many roles in signaling processes including smell, taste, sight, inflammation, and ...
This post was contributed by Patrick Miller-Rhodes from the University of Rochester Medical Center. You’ve probably heard of Forster Resonance Energy Transfer (FRET). Through the non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the ...
Until recently, there were no completely genetic tools that would allow researchers to label just a fraction of a single genetically-defined subset of cells. By labeling fewer cells in a population, it’s easier to visualize individual/non-overlapping cells. While transgenic ...
The ocean is full of striking visuals from fluorescent jellyfish, bioluminescent phytoplankton, and colorful corals, to name a few. What’s behind these sights are an abundance of biological molecules like chromoproteins, fluorescent proteins, and luminescent proteins. While ...
Part stable scaffold, part epitope binding region, all fused to a fluorescent protein. “Frankenbody,” like the infamous frankenstein, was constructed by grafting different parts together. By combining two optimized elements of an antibody probe, scientists can now more easily ...
