In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment? The three years flew by, but since then, scientists have adapted CRISPR nucleases for many more specific research needs. In this update, we will focus on the most recent ...
CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability to infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and viral transduction; but these methods aren’t stealthy enough to gain ...
In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more in-depth, but we hope that this blog series will help you find new CRISPR tools for your research! This ...
This post was contributed by Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at University of Rochester Medical Center. During development, complex genetic programs specify and assemble diverse arrays of neurons, forming the neuronal circuits that will later ...
While much of CRISPR research has focused on genome editing, numerous discoveries have been made using the Cas9 nuclease in the absence of genomic alterations. These studies utilize a catalytically inactive form of Cas9 known as dCas9 (Jinek et al., 2012). Like Cas9, dCas9 can ...
The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without changing them. Thus, it has been widely adapted for a wide variety of applications: base editing, CRISPR activation and inhibition, among others. Over the past few years, dCas9 ...
The vast majority of bacteria are undomesticated which limits the tools scientists can use to study them. For example, gene knockdown with CRISPR interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce CRISPRi machinery into ...
