Cas9 is the genome editing tool of choice for a number of model organisms: mammalian cells, yeast, drosophila, plants, worms, zebrafish, frogs, some bacteria; but not thermophilic (high heat loving) bacteria. Until recently the only available Cas9 proteins were isolated from ...
This post was contributed by guest bloggers Dominik Paquet and Dylan Kwart from Ludwig-Maximilians-University in Munich and Marc Tessier-Lavigne’s lab at the Rockefeller University in NYC. The CRISPR/Cas9 system is a versatile tool for precise gene editing in many organisms and ...
Note: After this blog was published, a bioRxiv preprint that questions the conclusion of inter-homologue recombination was released. This blog has not been updated in response to this paper. Any hint of CRISPR editing in human embryos has been met with a storm of media coverage. ...
This post was contributed by guest blogger Fredrik Wermeling, leader of a research group at the Centrum for Molecular Medicine (CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. It can be very time consuming to design 5 guide RNAs (gRNAs) targeting each of ...
Note: Cpf1 is also called Cas12a. There’s a new development for CRISPR-Cpf1 genome editing! A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing. For a few reasons, Cpf1 is a simplified system for editing multiple targets compared to Cas9. ...
Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at the Allen Institute for Cell Science. Learn more by visiting the Allen Cell Explorer at allencell.org and the Allen Institute website at alleninstitute.org. A classic challenge ...
We all know that in the lab there are often little tricks that are essential for experiments but that nobody talks about. After months of troubleshooting, those people who did not tell you that essential thing ask incredulously, “You seriously didn’t add 3 microliters of 5 mM ...
Colorful CRISPR technologies are helping researchers visualize the genome and its organization within the nucleus, also called the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent in situ hybridization (FISH) and chromosome ...
