Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the genome engineering world by storm owing to its ease of use and utility in a wide variety of organisms. While much of current CRISPR research focuses on its potential ...
This post was contributed by guest blogger Søren Hough, a Biochemistry PhD candidate at the University of Cambridge. One of the most important steps in the CRISPR experimental process is validating edits. Regardless of which CRISPR genome editing system you use, there remains a ...
This post was contributed by guest blogger, member of the Addgene Advisory Board, and Associate Director of the Genetic Perturbation Platform at the Broad Institute, John Doench. A genetic screening project can be a tremendous undertaking, producing a wall of results that can ...
Last updated Oct 7, 2020 by Gabrielle Clouse. This post was contributed by guest bloggers Marcelle Tuttle and Alex Chavez, researchers at the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 is an enormously plastic tool and has taken the scientific world by ...
This post was contributed by guest blogger, Aneesh Karve, CTO at Quilt Data. This post was originally published on the Quilt Genomics Blog and is republished here with permission. Quilt is a collaborative database for genomics. In this article, Quilt CTO Aneesh Karve, shows how ...
Update (November 18, 2016): Researchers from a variety of institutions recently reported their inability to recapitulate the results of Gao et al 2016 in a letter to Protein & Cell. Update (August 3rd, 2017) THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN ...
Note: Cpf1 is also called Cas12a. In 2015, Feng Zhang’s lab characterized two Cpf1 nucleases, distant cousins of well-known Cas9. Cpf1 cleaves DNA in a staggered pattern and requires only one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. Now, two new ...
Last updated on Oct 1, 2020 by Aliyah Weinstein. This post was contributed by guest bloggers, Wenning Qin and Haoyi Wang. CRISPR/Cas9 is revolutionizing the mouse gene-targeting field. Mice have long been extremely useful in the lab – they are relatively small and easy to work ...
