Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Born to a family in Malawi that pushed science education, Kingdom Kwapata grew up to be one of the best of the best students in his home country. He was selected as one of 600 scholars among 100,000 eligible to attend the University of Malawi where he focused on agricultural ...
Lentiviral vectors are one of the most popular and useful viral vectors in the lab. Advantages of lentivirus include a large genetic capacity and the ability to transduce both dividing and non-dividing cells. Lentiviral vectors are the vector of choice for many CRISPR ...
Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally this has been done with restriction enzyme digest; however colony PCR can accomplish the same thing in less time and for less money. The key steps to colony PCR are: 1) ...
Over 25 million people worldwide are currently infected with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral therapies such that the virus is undetectable in the blood. But the virus doesn’t completely disappear; it just hides in latently infected cells. To ...
This post was contributed by ATCC Outreach Scientist Nick Amiss. Cell culture in the present day Cell culture is a widespread tool used in the fields of oncology, virology, immunology, microbiology, and pharmacology (many of which are represented in Addgene's Special ...
The crisis of antibiotic resistance is upon us, and the world is unprepared. Each year in the United States, two million people will be infected by antibiotic resistant bacteria. Even when researchers develop new antibiotics, the onset of resistance is swift, as few as five ...
In a previous post from our Plasmids 101 series, we learned how the Cre-loxP recombination system can be used to induce site-specific recombination events, and that the orientation of the flanking loxP sites directs the Cre recombinase to invert, translocate, or excise a DNA ...
