Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Note: Cpf1 is also called Cas12a. There’s a new development for CRISPR-Cpf1 genome editing! A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing. For a few reasons, Cpf1 is a simplified system for editing multiple targets compared to Cas9. ...
Even before fluorescent proteins (FPs) came into wide use, there were a variety of ways to monitor cell, organelle, and protein localization. For instance, you might dye your cells and look at them under a microscope, fractionate samples to isolate particular organelles and ...
This is the second half of a two-part interview with Vini Mani and Amy Gilson from Science in the News (SITN) at Harvard University. There are tons of ways you can get involved in science communication. In this second half of our conversation with Vini Mani and Amy Gilson from ...
Fluorescent proteins (FPs) offer scientists a simple yet powerful way to tag cellular proteins and investigate protein localization, interaction, and expression. However, one caveat of FP-protein fusions (FP-chimeras) is that they undergo normal protein turnover. FP-chimeras are ...
This post was contributed by guest blogger, Stephanie Hays, a scientist with a passion for photosynthetic communities, microbial interactions, and science education. Disclaimer: The views presented in this article are those of the author do not represent a formal stance taken by ...
This post was contributed by guest bloggers Samantha Zyontz and Neil Thompson from the MIT Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed nearly 2,400 citations in its first four years (1). In addition to publishing in ...
This post was contributed by guest blogger Joe Mellor, Founder and CEO of seqWell Inc. Plasmids and PCR products are the bread and butter of molecular biology labs the world over. Scientists have traditionally used Sanger sequencing to validate these constructs, as the ...
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