Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Addgene has distributed over 800,000 plasmids since 2004. In that time, our lab has gotten a lot experience in processes including automated vial filling, accurate tube-labeling, quick plate pouring, and much more. We didn’t want to keep the knowledge we’ve gained along the way ...
Blugene and I represented Addgene at the recent Keystone meeting on Precision Genome Editing with Programmable Nucleases. Check out #KSgenome on Twitter if you missed our live updates!
This post was contributed by guest blogger Matthew J. Niederhuber, a graduate student at UNC Chapel Hill. Chromatin immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method for mapping where a protein binds genome-wide, and has been widely ...
This post was contributed by Kyle Hooper at Promega. Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the lab, if you read a paper and saw an interesting construct you wished to use, you could either make it yourself or you could ...
In today’s podcast, we sit down with Wei Leong Chew, a researcher at the Genome Institute of Singapore who recently started his own lab. We discuss some of the joys and difficulties of getting a lab up and running, and learn a little bit about what it was like for Wei Leong to ...
In this post of the Careers in Science Communication blog series, you’ll hear from Susan Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center.
We’ve talked a lot about the quality control process at Addgene by introducing our new sequencing partner seqWell and going into detail about how we use next generation sequencing results to perform quality control on deposited plasmids. We’ve also talked about how our new ...
