Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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The field of induced pluripotent stem cells (iPSCs) has been around for 10 years. In that time, scientists have used almost all available approaches for generating iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell ...
In this episode of the Addgene podcast, we sit down with Addgene’s Founders - Melina Fan, Ken Fan, and Benjie Chen. We learn what it was like to get Addgene off the ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what it ...
This post was contributed by guest blogger Courtney Price, the Education & Outreach Specialist for the Arabidopsis Biological Resource Center and the Center for Applied Plant Sciences at The Ohio State University. Established in 1991, the Arabidopsis Biological Resource ...
Antibodies are a go-to tool for detecting a protein of interest in cells and tissues. Although antibody production is well established, it’s also a process that’s difficult for individual labs to complete. The nanobody based RANbody platform from the Sanes Lab overcomes this ...
CRISPR genome editing has made it easier to create knockout alleles in a variety of species, including the standard laboratory mouse. It’s also made targeted insertions relatively simple in C. elegans and bacteria. But CRISPRing typical mouse models, including creating ...
In order to bind DNA, Cas9 and other CRISPR enzymes require a short PAM sequence adjacent to the targeted sequence at the locus of interest. SpCas9’s 3’ NGG PAM occurs frequently in GC-rich genomes, but a PAM is not always available near the locus you’d like to modify. To tackle ...
Guest blogger Todd Waldman, Professor at Georgetown University, contributed to this post. Adeno-associated viruses (AAVs) make fantastic gene delivery vehicles for episomal gene expression and are particularly useful for gene delivery to the nervous system. For many years they ...
