By Tyler Ford
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This post was contributed by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs. When do you need a high fidelity ligase—and when is an alternative ligase a better choice? And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes ...
Many of us take comfort in the fact that it’s often not quantity, but quality that really matters. Well, it turns out this isn’t the case for using AAV. When it comes to infecting cells, titer, the amount of virus used, really does matter. (*psst*, quality definitely also ...
Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically require secondary antibodies, frequently of the mouse or rabbit variety. While antibodies certainly aren’t “broken,” their production does require continued animal ...
As a tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, is to find new ways to make lab processes more efficient. Being in the Boston area, we get many opportunities to learn from other biotech companies at workshops and can use what ...
Addgene has distributed over 800,000 plasmids since 2004. In that time, our lab has gotten a lot experience in processes including automated vial filling, accurate tube-labeling, quick plate pouring, and much more. We didn’t want to keep the knowledge we’ve gained along the way ...
Blugene and I represented Addgene at the recent Keystone meeting on Precision Genome Editing with Programmable Nucleases. Check out #KSgenome on Twitter if you missed our live updates!
This post was contributed by guest blogger Matthew J. Niederhuber, a graduate student at UNC Chapel Hill. Chromatin immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method for mapping where a protein binds genome-wide, and has been widely ...
