Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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RNA-editing Cas13 enzymes have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock down RNA without altering the genome, but Cas13s have higher on-target specificity. New work from Konermann et al. and Yan et al. describes new Cas13d enzymes that ...
One way to define a protein’s purpose is by its protein-protein interactions (PPIs). These interactions are often modeled as binary relationships, i.e. protein A interacts with protein B; but proteins are social biomolecules. They can be part of multiple dynamic and overlapping ...
Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. Below you'll find ...
A recent survey of PhDs found that many researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the lab and by offering ...
The field of induced pluripotent stem cells (iPSCs) has been around for 10 years. In that time, scientists have used almost all available approaches for generating iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell ...
In this episode of the Addgene podcast, we sit down with Addgene’s Founders - Melina Fan, Ken Fan, and Benjie Chen. We learn what it was like to get Addgene off the ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what it ...
This post was contributed by guest blogger Courtney Price, the Education & Outreach Specialist for the Arabidopsis Biological Resource Center and the Center for Applied Plant Sciences at The Ohio State University. Established in 1991, the Arabidopsis Biological Resource ...
