By Guest Blogger
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All plasmids rely on their host cell's replication machinery in order to replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA replication is initiated at the ori and may or may not be synchronized with the replication of the ...
This year marks Addgene's 20th anniversary! We've been celebrating throughout the year, and on Sunday, June 9th, we hosted our 20th anniversary party for all Addgenies to celebrate together. We were even able to have many of our remote workers travel to Addgene's headquarters in ...
When you are running flow cytometry, you’ll need various controls to help you set up and analyze your samples. While you are probably familiar with the basics of controls in experimental design, you’ll need a few controls specific to flow as an application. These controls will ...
Have you ever designed a CRISPR guide RNA and wondered why it is limited to only 20 bases, or why it’s so important to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever more ubiquitous tool for genome engineering, and studying its ...
If you’re depositing a pooled library with Addgene, you may be surprised to learn that we ask for an amplification protocol with your deposit. This is because repeated amplifications of pooled libraries can lead to issues such as recombination and loss of plasmid diversity, so ...
When analyzing your cells using flow cytometry, you are typically measuring the presence or absence of certain markers on the surface or the inside of your cells. While proteins themselves can emit intrinsic fluorescence when excited with ultraviolet (UV) light, they do so via ...
Every few months we highlight some of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.
