By Rachel Leeson
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Western blots can be tricky to get right. If you’ve thoughtfully done the technical design of your blot, but are still finding yourself having issues, you’re in the right place! In this post, we’ll talk about western blot optimization and troubleshooting.
If you’re using antibodies in your research, you've probably found yourself staring at a browser full of tabs, each open to a different antibody option. Or you may find yourself with only two options, but very little data on which might work in your application. It can be quite ...
Antibody validation is to confirm (or refute) that the antibody is selectively detecting the target-of-interest in your assay and sample-of-interest. The approaches available broadly map onto the five pillars of antibody validation (see: Uhlen et al., 2016). In this post, we ...
Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot Abi? If so, you are not alone. Full-sized antibodies dominate the world of research affinity reagents, and for good reason. However, sometimes you want a tool that is a little ...
If you’ve ever been looking for just the right CRISPR vectors on Addgene and found instead ones that were… pretty close, or at least close enough, you’ve found yourself with a common dilemma. Request the vectors you can find and use them as-is, saving time and effort but risking ...
Every few months we highlight some of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.
If you’ve ever run a western blot, or thought about running one, you’ll know there’s a lot of choices to make when designing the experiment. What detection method? What membrane? What should you block with?
