By Joanne Kamens
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Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel. CRISPR genome editing has quickly become a popular system for in vitro and germline genome editing, but in vivo gene editing approaches have been limited by problems with Cas9 delivery. ...
If you’re cloning a plasmid, you’ll need a way to find the needle in the haystack: the one perfect clone that contains the plasmid you’re looking for out of the many cells that don’t. One way to begin the search is by using selection strategies, where only cells that have gained ...
Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy to target multiple loci - a concept called multiplexing. Since CRISPR is such a robust system, editing or labeling efficiency doesn’t usually change when you add multiple ...
The Rare Genomics Institute BeHEARD (Helping Empower and Accelerate Research Discoveries) Challenge provides grants for rare disease research. This year is our fourth year working with the Rare Genomics Institute by providing plasmid-based tools for rare disease research. ...
This blog post was originally written by Caroline LaManna, published Mar 8, 2016. The updated and expanded version by Nyla Naim was published Sept 3, 2020. Scientists around the world have been making major improvements to CRISPR technology since its initial applications for ...
Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging capacity is limited to ~4.7kb, or roughly half the packaging limits of lentiviral and adenoviral vectors. While many transgene will fit within this limit, some like prime ...
This post was contributed by Patrick Miller-Rhodes from the University of Rochester Medical Center. You’ve probably heard of Forster Resonance Energy Transfer (FRET). Through the non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the ...
