CRISPR Guide Update

By Alyssa Shepard

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Resistance plasmids are depicted with a shield for defense. Col plasmids are depicted with a sword for offense. Virulence plasmids are depicted with a flask of toxins for infection. Degradative plasmids are depicted with a trash can on a dinner plate for hardiness. Fertility plasmids are depicted with a handshake for sharing. Cryptic plasmids are depicted with a question mark for mystery.
AAV infection of retinal neurons.
Schematic of multicomponent assembly with SLIC.
Two graphs, of fluorescence versus time and the residual (F - F_fit)/F_fit versus time, with vertical bands alternating gray and white every 5 seconds. The signal follows a prominent sawtooth pattern, decreasing during the gray period and recovering during the white period. Bottom panel (residuals) indicates ΔF/F reaches 75% and decreases slightly each cycle, but still reaches over 50% after seven cycles.
Exposed membranes showing a protein gradient with a primary antibody at two different dilutions.

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