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Synthetic promoter AAVs for cell-type specific expression in retinal cells

Posted by Beth Kenkel on Mar 12, 2020 9:15:00 AM

Adeno-associated virus (AAV) is a popular tool for gene delivery, but it has a drawback: how do you ensure your gene goes where you want it to? Knowing that a gene is expressed in a particular cell type is important not only for translational research, such as gene therapy, but also basic research. To improve cell-type specificity of AAV, work has focused on modifying the outside protein shell, or capsid, of the virus so that it only enters and delivers it cargo to certain cell types. 

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Topics: Viral Vectors, Cell Tracing, AAV

What's New in CRISPR - March 2020

Posted by Jennifer Tsang on Mar 10, 2020 9:15:00 AM

In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more in-depth, but we hope that this blog series will help you find more new CRISPR tools for your research!

This time:

  • Optogenetic repression in yeast
  • Base editing
  • CRISPRi in Caulobacter crescentus
  • Multiplexed gRNA for human pluripotent stem cells
  • CRISPR-based DNA demethylation
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Topics: CRISPR, Other CRISPR Tools

Celebrate Open Data Day with Addgene’s AAV Data Hub!

Posted by Angela Abitua on Mar 5, 2020 9:15:00 AM

We’re big fans of all forms of research sharing and with Open Data Day 2020 coming up on March 7th, what better way to celebrate open data sharing than with Addgene’s AAV Data Hub! We launched this pilot in 2019 as an open science platform for scientists using Addgene AAV viral preps to easily find and share useful technical data. These data focus on experimental design and usage of these tools, which aren’t always easy to find in publications, so this is a much-needed community resource. Instead of painstakingly searching for experimental details in research articles, scientists can use the AAV Data Hub to quickly find details they need for planning their next experiments using a specific AAV tool. They can search the database by certain features like serotype, promoter, or tool type and each entry contains an image along with key experimental details (e.g. titer, injection site, animal strain used). Similar to the goodwill of Addgene plasmid depositors, this growing resource of over 100 entries is driven by the dedicated community of data-contributing researchers who use AAV tools.

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Topics: Viral Vectors, Addgene’s Viral Service

Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

Posted by Various Addgenies on Mar 3, 2020 9:15:00 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

 

 

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Topics: Hot Plasmids, Other Plasmid Tools, Plasmids

How Do I Place an Addgene Order Using a PO Number?

Posted by Various Addgenies on Feb 27, 2020 9:15:00 AM

By Natalie Mortenson and Laura O'Regan

Ordering jeans online from your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene, we try to make the process as smooth and simple as possible.

We are the Customer Support Specialists here at Addgene. If you’ve called our customer service line or sent an email to help@addgene.org, you’ve likely talked to one of us! Today we’re discussing some of our most frequently asked questions related to the ordering process:

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Topics: Inside Addgene, Using Addgene's Website

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