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A media supplementation strategy to improve AAV yields

Posted by Meghan Rego on Dec 13, 2018 8:14:12 AM

By now you have probably heard about Addgene’s viral service but did you know that Addgene also performs independent research? Since launching the viral service in 2016 Addgene’s research team has been actively investigating ways to improve viral vector production. Whether redesigning viral packaging plasmids, investigating different viral production strategies or developing novel methods for data analysis, our team’s goal is to provide scientists with new tools and protocols that they can easily incorporate into their own labs. This week we are pleased to announce the submission of the results of our first study which can be accessed here at bioRxiv.

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Topics: Viral Vectors

Early career researcher toolbox: social media for scientists

Posted by Beth Kenkel on Dec 11, 2018 7:46:17 AM

Before I started writing for the Addgene blog, sharing Chemistry Cat memes was how I used social media as a scientist. I mean, I had a LinkedIn page and a Twitter handle, but I wasn’t using them to my professional advantage. It wasn’t until I wrote a blog post about a research paper that explored how scientists connect on Twitter that I realized 1) a lot of scientists are using social media professionally, and 2) I needed to start using Twitter.

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Topics: Networking, Science Communication

Hot Plasmids December 2018 - C. elegans RNAi, TurboID, detecting viral outbreaks, and protein binding specificity tools

Posted by Various Addgenies on Dec 6, 2018 8:34:06 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

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Topics: Hot Plasmids

Winter is here and it's time to #DeckTheLab

Posted by Jennifer Tsang on Dec 4, 2018 9:01:57 AM

Winter is upon us and we’re getting into the holiday spirit here at Addgene. We’ve adorned our walls with snowflakes, put out some hot chocolate, and added some winter cheer to the lab.

Now it’s your turn! Show us your lab holiday decorations by participating in our annual #DeckTheLab contest. Get creative and build a pipette tip tree or go all out and create a massive wall mural. Snap a photo of your lab holiday decor and tag it with #DeckTheLab and @Addgene. This year, we’re opening up the contest to Twitter and Instagram.

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Topics: Fun, Inside Addgene

CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

Posted by Benoit Giquel on Nov 29, 2018 8:53:18 AM

Before being adapted by scientists to edit the genome of virtually any organisms on this planet, CRISPR-Cas systems were merely adaptive immune systems that provide bacteria protection against infectious agents. Several enzymes behind this immunity have already been discovered and studied but it is only the tip of the iceberg as it has been predicted that many others are still unknown.

In this quest to discover new and maybe more efficient Cas systems, Jennifer Doudna’s lab analyzed metagenomic datasets to try to determine whether simpler and maybe smaller Cas systems could exist in nature.

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Topics: CRISPR

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