By Margo R. Monroe
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“Learning is not attained by chance, it must be sought for with ardor and attended to with diligence.” - Abigail Adams, 1780 There are potential mentors all around you. This third article in the Addgene Blog Mentoring series will cover 2 of the 7 mentoring questions I set out to ...
Resistance to antibiotics is a widely used tool in molecular biology, yet scientists rarely stop to think about how much easier it makes our lives. Plasmid transformation into E. coli is a fairly inefficient process– just 1 out of 10,000 cells on average! Without some means of ...
We perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We do this by making our own competent cells and using a little-known reagent for streamlining the transformation step. Read on to find protocols and tips that you can use in your ...
A few years ago, researchers introduced what’s been called a major breakthrough in cloning technology with the Golden Gate method. For the first time, it was possible to quickly and efficiently assemble a large number of building blocks with just two or three inexpensive ...
Which graduate student hasn’t been asked the question: “Academia or industry?” Once academia was the clear answer with a well-defined path to professorship. But recently a downward trend in funding and space in academic research labs has more students looking for other options. ...
A scientist-in-training will spend 10 or more years with a small number of formal advisors learning how to be a scientist. It is shocking how little pre-work most PhD students and postdocs do to ensure the advisors they choose will be ones that help them succeed after the ...
Addgene was founded 10 years ago today. In that time, Addgene has shipped over 350,000 individual plasmids to 5,000 different research institutions. This has given us a unique window into technology trends in the life sciences. In this post, we'll give you an inside look at the ...
