Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Lentiviruses are useful and efficient tools to introduce your gene of interest into cells. Unlike gamma-retroviruses that can only infect dividing cells, lentiviruses can infect dividing and non-dividing cells. Addgene has an extensive collection of lentiviral plasmids created ...
After joining a lab, the focus shifts to choosing an unexplored and impactful research topic that aligns with your interests. We've outlined some important factors to consider and summarize helpful advice from the successful PIs who were previously featured in "9 Tips to Achieve ...
It is probably good news for our environment that more people are paying attention to living a “green” life and creating less waste. Most communities seem to have recycling programs and it seems to me that most Addgenies make an effort to avoid plastic bags and to conserve ...
Choosing a lab can be a major decision. A science trainee will spend 4-7 years working for one person and with a group who all strive for a common big-picture goal. It is worth doing some some serious pre-work to find a lab that will be a good fit for you and your career ...
Once you've gotten to know what a plasmid is in general, it's time to take a look at some of its parts. Elements such as the antibiotic resistance gene and the origin of replication are necessary to replicate plasmids and make sure the cells maintains it. Here, we'll cover the ...
This blog post is the first in a series that will feature advice for students, postdocs, and young Principal Investigators (PIs). We've interviewed Addgene depositors who are at various stages in their careers to get advice on choosing a lab, picking your research topic, ...
If you start poking around on Addgene’s Fluorescent Protein Guide to In Vivo Imaging, you’ll pretty quickly notice the name Vladislav Verkhusha popping up again and again, and for good reason. We all know scientists have used fluorescent proteins to observe what’s happening ...
