Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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If you start poking around on Addgene’s Fluorescent Protein Guide to In Vivo Imaging, you’ll pretty quickly notice the name Vladislav Verkhusha popping up again and again, and for good reason. We all know scientists have used fluorescent proteins to observe what’s happening ...
Although plasmids do not naturally exist in mammals, scientists can still reap the benefits of plasmid-based research using synthetic vectors and cultured mammalian cells. Of course, these mammalian vectors must be compatible with the cell type they are tranfected into – a ...
This article was originally published on the Society for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood. Work/life balance. Is it truly possible to bring all aspects of our lives under control? Balance is a myth and should not even be the real ...
As Kendall mentioned in Tuesday's blog post, keeping up with the newest CRISPR technologies and their applications can be exhausting. A quick search for "CRISPR", short-hand for Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed returned 728 articles ...
If any of you are finding it hard to keep up with the news on CRISPR, there's a pretty good reason for that. Lately, significant advances in the understanding and application of CRISPR/Cas9 technology are coming along at a fast and furious pace. In December, as we've blogged ...
This is the fifth and final post in the Addgene Blog Mentoring for Scientists Series. The entire series and additional resources can be downloaded in E-Book format at the end of this post. If you have been following the posts in this Mentoring for Scientists series, you have: ...
As MIT's Ed Boyden explains it, his goal is "to understand the brain at a level of abstraction that enables the engineering of its function." Once scientists can do that, they will not only understand how the brain works, but also how to fix it when things go awry. To reach that ...
