By Caroline LaManna
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The following post was contributed by the Developmental Studies Hybridoma Bank. Antibodies are the most widely used class of protein-binding reagents. In the lab, their binding specificity allows scientists to target proteins of interest for labeling, quantification, ...
Over the past decade there has been a shift in how science is perceived - where once science was only seen as a serious academic subject, it is now being appreciated on an aesthetic level as well. Beautiful microscope images which traditionally languished in the pages of thick ...
CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's Silvana Konermann, the first generation of CRISPR-Cas9 plasmids were not designed with gene activation in mind. “We had not managed to create a system to allow us to ...
This is the fourth in a 5 part series on Management for scientists. Once you are responsible for managing others you will only be successful in your role if you become a master at delegating tasks and responsibilities. The manager is not expected to DO all the work – she is ...
The following post was contributed by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical School. Addgene is proud to present a video reprint of the CRISPR article "Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9" from the ...
In a previous Plasmids 101 blog, we reviewed the salient features of several popular strains of E. coli for DNA propagation. While great for cloning purposes, these E. coli strains are not usually well suited for recombinant protein expression. Many challenges can arise when ...
This is the third in a 5 part series on Management for scientists. Feedback is help and it should result in authentic assistance for the recipient to become more effective and successful. Giving timely and useful feedback is an absolutely required aspect of being a successful ...
