Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Since the first research applications of GFP were published in the 1990s, biologists have spent a lot of time making things glow. Chances are you’ve used a GFP derivative to conduct subcellular localization studies or make a reporter construct. Fluorescent proteins (FPs) are ...
As Eric Mendenhall of the University of Alabama in Huntsville explains it, a major goal in his laboratory is to understand the function of the non-coding portion of the genome. Mendenhall and Richard Myers of HudsonAlpha (where Mendenhall is also an adjunct faculty member) have ...
The following post was contributed by Derek Jacoby from Makerspace Victoria, CA Over the decades science has become increasingly restricted to academic and industrial labs, but recently there has been a counter movement by the public to access basic equipment and to become ...
As we get closer to the start of another academic year, graduate students and post-docs alike are wondering where the time has gone. Are we any closer to graduating, publishing that key paper, or figuring out a career path? Many trainees are developing Individual Development ...
This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery at the University of Miami. The beginning of this century has seen some major advances in light microscopy, particularly related to the neurosciences. These developments in ...
Expanding your network of relationships early and often is the most effective tactic a scientist in training can adopt to ensure opportunities in the future. Studies show that the majority of job offers arise as a result of existing professional (and personal) relationships. ...
In the summer of 2013, a remarkable nine papers describing CRISPR/Cas9 genome engineering methods for C. elegans were released, signaling a new era in C. elegans research. Homology directed repair (HDR), which enables insertion of custom genomic modifications, is very robust in ...
