By Emma Markham
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Primary cells recapitulate the natural biology of a cell type of interest better than immortalized lines derived from the same cell type; however, their usage has been limited by technical problems. For instance, it’s much more difficult to introduce a gene of interest into ...
Protein purification can be one of the most stressful lab activities. Working with proteins requires a substantial amount of properly folded, relatively pure protein, but getting to this stage is often much easier said than done. As reviewed in our Plasmids 101 series, proteins ...
Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate student organization Science in the News (SITN), we recently sponsored a first-time event called DayCon. DayCon is a one-day conference aimed at the general public that ...
Exciting news! Addgene recently rolled out a new feature on our plasmid pages - links to articles citing this plasmid. Now you can learn how a plasmid has been used by multiple labs and see what experimental systems it has been validated in. If a plasmid's Addgene ID # has been ...
This post is part of our ongoing Plasmids 101 series. Plasmids 101 will provide you with an overview of general molecular biology knowledge and techniques. If you are interested in reading more, you can find the rest of the Plasmids 101 posts here. Now that we have covered ...
In the short time since its development, CRISPR/Cas9 genome editing has been used to study the effect of gene knockout in vivo and in vitro, as well as to insert targeted mutations through homologous recombination. To further increase the utility of CRISPR/Cas9, it will be ...
This post was contributed by Kevin Esvelt, a Wyss Technology Development Fellow at the Wyss Institute and Harvard Medical School. Scientists making transgenic organisms with Cas9 should be aware of the potential hazards of creating “gene drives” capable of spreading through wild ...
