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Addgene’s plasmids are used with a wide variety of restriction enzyme-based cloning methods. Each method has its own pluses and minuses, but Golden Gate cloning has been especially useful within both the synthetic biology and genome engineering fields. We’ll walk you through how ...
Being an entrepreneur is difficult; it requires an immense amount of self-knowledge and an unwavering devotion to seeing your ideas become something tangible. When you think of entrepreneurship in the 21st century, who do you think of? Your mind is likely drawn to the Mark ...
This post was contributed by Jo Miller, founder and principal of J. Miller & Associates, Inc., a national grant consulting firm, and Smartegrants, a grant professional development firm The path to a career as a Grant Professional has been a long and winding path for many in ...
This post was updated on Aug 20, 2020. What genes are important in your phenotype of interest? Many scientists study diseases for which the underlying genetic cause is not entirely known. Identifying which genes are important for a phenotype can lead to a wealth of additional ...
The Addgene Advisory Board consists of prominent members of the bioscience and tech transfer communities who help guide and promote Addgene in its mission to make research easier. Addgene is excited to announce that we’ve added John G. Doench, PhD to the Advisory Board. John ...
It seems that there’s a new CRISPR advance or technique published every week! One of the newest applications is a colorful system that uses fluorescently labeled Cas9 to label multiple genomic loci in live cells. While other systems can be used to label loci, such as ...
The follow post was contributed by guest blogger Juliet Moncaster Leadership skills are amongst the professional abilities we often hear that scientists should acquire during their PhD and postdoctoral training. Addgene executive director Joanne Kamens has written a 5-part blog ...
