Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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This post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software and the piñata effect Two years ago I was a part of a group (Biology of Host-parasite Interactions, Institut Pasteur, Paris) that changed genome editing in the malaria community ...
Bioblasts? Plasmagenes? In the 1940s and 50s, scientists were working to understand genetic cytoplasmic factors that could be transferred between cells. At the time, these extranuclear agents of heredity were thought of as everything from parasites, to symbionts, to genes and ...
This post was contributed by guest bloggers Erik L. Snapp and Lindsey M. Costantini. "You underestimate the power of the Dark Side." --Darth Vader in "Return of the Jedi" While Vader was referring to the evil side of a mystical "Force," this quote is equally applicable to many ...
Two hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively to screen for molecular interactions in the cell, including protein-protein, protein-DNA and protein-RNA interactions. The 1980s saw a flurry of discovery in the field of ...
The second Boston Symposium on the Future of Research will be held from 22-24 October. This blog has been contributed by guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses one of the six panel discussions to be held at the ...
Updated Aug 13, 2020.
Every plasmid sample enters Addgene the same way. A package is delivered by a mail courier and then the journey of transformation and storage begins. Some samples are submitted as bacterial colonies in petri dishes, but close to 80% of samples are received as DNA in a ...