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Meghan Rego

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Overcoming the challenges of lentiviral production

Posted by Meghan Rego on Aug 7, 2018 8:31:12 AM

While lentiviral vectors are popular gene delivery tools, producing lentivirus, can pose certain challenges. Whether choosing a system that is the best fit for the experiment, trying to produce virus of a usable titer, or fine-tuning selection and expression in your target cell line, researchers often find themselves faced with a roadblock. In this post, we will provide an overview of some of the common challenges associated with producing and using lentivirus and offer some tips and tricks for overcoming these hurdles.

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Topics: Viral Vectors, Techniques, Lab Tips

Getting the Most from Your Lentiviral Transduction

Posted by Meghan Rego on Apr 7, 2016 10:30:00 AM

Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express a gene of interest. While the general approach for using lentivirus, infect and select, seems simple, in actuality, many find using lentivirus to be time consuming, difficult, and lacking in reproducibility. Read on for some tips for getting the most out of your lentiviral transduction experiments.

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Topics: Lab Tips, Viral Vectors

Tips for Titering Your Lentiviral Preps

Posted by Meghan Rego on Mar 15, 2016 10:30:00 AM


The day has arrived; you’ve painstakingly cared for your packaging cell line, prepped your DNA, transfected and harvested your lentivirus. Now it’s time to move ahead with your infection and make your stable cell line. While we’ve all experienced the pressure to move a project forward, transductions should not be rushed into. Before you start any transduction, you should always titer your virus - that is, determine the amount of virus you actually have in your prep. Taking time to properly titer your virus will not only ensure that your infection is designed in the best possible way, but it may also save you time in the long run. Read on for an overview of the titering options as well as the benefits and drawbacks of different methods (for comprehensive protocols for all of the methods discussed here refer to
Kutner et. al.).

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Topics: Techniques, Viral Vectors

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