Much of today's biological research requires a close examination of specific proteins within a system. This can be pretty complicated given that a single cell has tens of thousands of proteins functioning in a variety of ways. How do scientists focus on the activity or function ...
Picture this: you’ve been assigned an exciting new project aimed at understanding how a critical cellular pathway is regulated. You’ve read all the background papers you could get your hands on, formulated a hypothesis, and planned out your key experiments. Unsurprisingly, many ...
Immunoprecipitation (IP) uses immobilized antibodies, or immunoglobulins, to isolate a specific protein out of a complex mix. Using this technique, users can look for the presence or absence of a protein, determine if a protein is up or downregulated, examine a protein’s ...
Have you ever wanted to measure expression of your protein of interest in a single cell? Or perhaps, you need to analyze a specific subset of cells in a complex population. Have you spent hours in the biosafety cabinet with cloning rings or following labor-intensive limiting ...
You’ve gotten the plasmid encoding your protein of interest from Addgene, transfected it into your target cells, now what? How can you tell if the protein you are so keen to study is expressing in your cells? Immunoblotting or simply the western blot, or western, is one of the ...
One of the main tenets of our quality control (QC) mantra is to do our utmost to ensure that scientists are receiving the exact materials that they think they are. To address this, several years ago we partnered with Seqwell to establish a next-generation sequencing (NGS) QC ...
By now you have probably heard about Addgene’s viral service but did you know that Addgene also performs independent research? Since launching the viral service in 2016 Addgene’s research team has been actively investigating ways to improve viral vector production. Whether ...
While lentiviral vectors are popular gene delivery tools, producing lentivirus, can pose certain challenges. Whether choosing a system that is the best fit for the experiment, trying to produce virus of a usable titer, or fine-tuning selection and expression in your target cell ...
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