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Jennifer Tsang

Jennifer Tsang is the Science Communications and Marketing Coordinator at Addgene.

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What's New in CRISPR - September 2019

Posted by Jennifer Tsang on Sep 10, 2019 8:56:17 AM

In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more in-depth, but we hope that this blog series will help you find new CRISPR tools for your research!

This time:

  • GeneWeld vectors to create knock-ins
  • CasX
  • Drug inducible CRISPR/Cas9 activation
  • Ecoli genome-wide inhibition library
  • CRISPR knockout libraries for cancer research
Read More >

Topics: CRISPR, Other CRISPR Tools

Plasmids 101: Positive and Negative Selection for Plasmid Cloning

Posted by Jennifer Tsang on Aug 22, 2019 8:43:39 AM

You’ve worked hard to purify your gene of interest, get it into your plasmid backbone, and zap the mixture of DNA into cells. Unfortunately, not every cell successfully takes up plasmid DNA. Among those that do, some now have plasmids that contain your gene of interest, but others will uptake plasmid backbones that re-ligated back on themselves.

Therefore, your cloning strategy needs to identify cells containing the plasmid construct you’re seeking. Fortunately, there are many ways to do this involving positive selection, negative selection, and/or screening. We’re focusing on positive and negative selection in this blog post, but don’t worry, we’ll cover screens in a future post.

Read More >

Topics: Plasmids 101, Plasmid Cloning, Plasmids

Plasmid Tools for Microbiome Studies

Posted by Jennifer Tsang on Jun 27, 2019 8:25:58 AM

Microbiome studies have traditionally fallen into studies of who’s there and what are they doing. To address these questions, biologists often use next-generation sequencing. Sequencing the 16S rRNA reveals the identity of the organisms present while sequencing of all transcripts gives clues into what the microbes are doing.

But aside from sequencing, scientists can also study the microbiome by using engineered genetic tools and reporter microbes. Let’s take a look at a few of these methods below.

Read More >

Topics: Microbiology

Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

Posted by Jennifer Tsang on Jun 4, 2019 9:02:29 AM

Fluorescent proteins have enabled scientists to pursue creative research avenues previously unavailable to them. With these tools it’s now easy to monitor protein expression, localization, and protein-protein interactions. Beyond these common applications, researchers are finding new ways to apply fluorescent proteins everyday. 

Read More >

Topics: Fluorescent Proteins

What's New in CRISPR - May 2019

Posted by Jennifer Tsang on May 28, 2019 8:49:09 AM

In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more in-depth, but we hope that this blog series will help you find new CRISPR tools for your research!

This time:

  • Ecoli genome-wide CRISPR inhibition
  • Covalent tethering of DNA template to Cas9
  • SECURE base editors
  • Nme2Cas9
  • CRISPR interference in Candida albicans
Read More >

Topics: CRISPR, Other CRISPR Tools

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