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Jennifer Tsang

Jennifer Tsang is the Science Communications and Marketing Coordinator at Addgene.

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The time and cost required to make a plasmid

Posted by Jennifer Tsang on Sep 11, 2018 9:48:42 AM

Have you ever wondered how long it takes to make a plasmid? Or have you thought about how much total time you spend in the lab cloning before you can start on your experiment? What about all the reagents you need to order? Sometimes, it feels like an eternity of cloning, waiting, and repeating before you can finally dive deep into experiments.

Since one million plasmids shared is upon us at Addgene, we started wondering, just how much time and money do researchers spend cloning? How much does material sharing speed science?

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Topics: Lab Tips, Plasmid Cloning

Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Posted by Jennifer Tsang on Sep 4, 2018 8:53:28 AM

Researchers express genes of interest from plasmids in order to study gene function or to engineer cells for specific purposes. Unfortunately, plasmid copy numbers vary within cell populations and over time resulting in variable gene expression that can impact observed phenotypes. Factors such as the growth medium, growth temperature, and growth rate can all impact plasmid copy number in a cell.

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Topics: Plasmid Technology, Plasmid Elements, Synthetic Biology

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