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Jennifer Tsang

Jennifer Tsang is the Science Communications and Marketing Coordinator at Addgene.

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Six Spooky Science Stories and Halloween at Addgene

Posted by Jennifer Tsang on Oct 31, 2019 9:06:51 AM

Plasmid pumpkins, team costumes, and spooky science stories. Yes, please!

At Addgene, we love finding ways to incorporate science themes into our fun activities and Halloween is no exception. For the past 10+ years, Addgenies have formed teams, chosen a theme for the costume, and did their best to claim the costume contest trophy. We’ve had teams dressed up as plasmids, Blugene, and the Addgene ping pong table.

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Topics: Inside Addgene, Addgene News

Prime Editing: Adding Precision and Flexibility to CRISPR Editing

Posted by Jennifer Tsang on Oct 24, 2019 9:26:53 AM

There are over 75,000 pathogenic genetic variants that have been identified in humans and catalogued in the ClinVar database. Previously developed genome editing methods using nucleases and base editors have the potential to correct only a minority of those variants in most cell types. A new technique from David Liu’s lab at the Broad Institute could add more precision and flexibility to the CRISPR editing world.

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Topics: CRISPR, Cas Proteins, CRISPR gRNAs, Base Editing

We’re Going Viral This Week!

Posted by Jennifer Tsang on Oct 14, 2019 9:00:00 AM

We first “went viral” in 2016 when we introduced the viral service, and now we’re “going viral” again!

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Topics: Viral Vectors

What's New in CRISPR - September 2019

Posted by Jennifer Tsang on Sep 10, 2019 8:56:17 AM

In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more in-depth, but we hope that this blog series will help you find new CRISPR tools for your research!

This time:

  • GeneWeld vectors to create knock-ins
  • CasX
  • Drug inducible CRISPR/Cas9 activation
  • Ecoli genome-wide inhibition library
  • CRISPR knockout libraries for cancer research
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Topics: CRISPR, Other CRISPR Tools

Plasmids 101: Positive and Negative Selection for Plasmid Cloning

Posted by Jennifer Tsang on Aug 22, 2019 8:43:39 AM

You’ve worked hard to purify your gene of interest, get it into your plasmid backbone, and zap the mixture of DNA into cells. Unfortunately, not every cell successfully takes up plasmid DNA. Among those that do, some now have plasmids that contain your gene of interest, but others will uptake plasmid backbones that re-ligated back on themselves.

Therefore, your cloning strategy needs to identify cells containing the plasmid construct you’re seeking. Fortunately, there are many ways to do this involving positive selection, negative selection, and/or screening. We’re focusing on positive and negative selection in this blog post, but don’t worry, we’ll cover screens in a future post.

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Topics: Plasmids 101, Plasmid Cloning, Plasmids

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