The natural CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to target the genome of the invading phage pathogen. Over the past decade, CRISPR tools have leveraged such host-defense mechanisms to enable multiplex gene editing in a variety of ...
As a student at Northeastern University, you are expected to spend at least one semester (the majority spend two to three) working in your chosen field. This cooperative education experience (co-op) is what has brought me, a second year bioengineering student, to Addgene. I am ...
Did you catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted a question that didn’t get answered live - but he kindly took some time to address all your unanswered questions via text! (well, maybe not all of ...
Selectable transgene markers for plant transformation fall into two main categories: antibiotic/herbicide resistance and fluorescent proteins. Selection using resistance genes usually involves killing the untransformed seedlings or callus (undifferentiated plant cell culture), ...
This post was contributed by guest blogger Daniel M. Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase DNMT3A allows ...
This post was contributed by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College of Medicine. Several sets of near-infrared fluorescent proteins (NIR FPs) and biosensors have been created recently. As developers of many of these probes, we decided to ...
This post was contributed by guest blogger Eleanor Wang, a Research Specialist in Patrick Hsu's lab at UC Berkeley. Most of the scientists I’ve met began doing research in their undergraduate years. We each have a unique “origin story,” but taking the first step into the world ...
This post was contributed by Ignacio Amigo and María Hernández Sánchez from Crowdfight. In March 2020, while most of the world was locked down, a South African company wanted to test if a type of antibodies from an Andean mammal called alpaca could neutralize the SARS-CoV-2 ...
