This post was contributed by guest blogger Talley Lambert, a Research Associate at Harvard Medical School. The need for a community fluorescent protein database As recognized by the 2008 Nobel Prize, fluorescent proteins (FPs) have become one of the most indispensable tools in ...
This article was contributed by Jessica Roginsky, Scientific Support Lead at Synthego. Article source: Step-by-Step Guide for Analyzing CRISPR Editing Results with ICE on Synthego’s blog. CRISPR-based genome engineering has revolutionized the gene editing field by making ...
This post was contributed by guest blogger Courtney Price, the Education & Outreach Specialist for the Arabidopsis Biological Resource Center and the Center for Applied Plant Sciences at The Ohio State University. Established in 1991, the Arabidopsis Biological Resource ...
This post was contributed by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs. When do you need a high fidelity ligase—and when is an alternative ligase a better choice? And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes ...
This post was contributed by guest blogger Matthew J. Niederhuber, a graduate student at UNC Chapel Hill. Chromatin immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method for mapping where a protein binds genome-wide, and has been widely ...
This post was contributed by Kyle Hooper at Promega. Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the lab, if you read a paper and saw an interesting construct you wished to use, you could either make it yourself or you could ...
This post was contributed by guest blogger Jon Backstrom, a biochemist in the Vanderbilt Eye Institute and Tonia Rex's lab. A common strategy to determine the binding kinetics of a purified protein involves immobilization on a solid support. This allows washing away of unbound ...
This post was contributed by guest blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize protein turnover within a neuron? For my PhD I studied a synaptic protein that is linked to neurodegeneration. The level of this protein is decreased in Alzheimer’s ...
