Emily P. Bentley

Emily P. Bentley is a Blog Writer at Addgene. She earned her PhD in molecular biology and biophysics from Scripps Research, and she loves supporting open science and learning about new research.

Blog articles by Emily P. Bentley

A cartoon plasmid at the center of the image has arrows leading to many possible host species: gram negative bacteria, gram positive bacteria, yeast, and mammalian cells.
A smiling Blugene holding a cartoon western blot.
The nucleobases are shown with arrows describing conversions between them. In step one, an adenosine deaminase converts adenine to hypoxanthine (the nucleobase component of inosine); this is catalyzed directly by the base editor. Next, base excision of hypoxanthine (also by the base editor) could be repaired in two different ways by the cell, shown by an arrow that splits into two outcomes. The repair pathway leading to cytosine is favored by ACBEs, while the repair pathway leading to thymine is favored by AYBEv3 + Polη.
The nucleobases are shown with arrows describing conversions between them. In step one, a cytosine deaminase converts cytosine to uracil; this is catalyzed directly by the base editor. Next, base excision of uracil (also by the base editor) is repaired in two different ways by the cell, shown by an arrow that splits into two outcomes. Repair in E. coli leads to adenine, while repair in mammalian cells leads to guanine.
A cartoon of a prime editor with two different edit sequences. The DNA sequences are shown with one strand edited and a 5′ DNA flap, before heteroduplex resolution and DNA repair.  The first edit has an unchanged PAM. This DNA is shown connected to the prime editor by a two-way arrow, indicating that the editor can re-bind. Re-nicking is represented by scissors and would remove the newly edited DNA.  The second edit has an altered PAM. A one-way arrow leads from the prime editor to this edit, indicating that the changed PAM prevents the editor from re-binding.
A decorative image depicting the protein structure of Top7 with a cartoon happy face wearing a Nobel Prize on a ribbon.

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