Emily P. Bentley is a Blog Writer at Addgene. She earned her PhD in molecular biology and biophysics from Scripps Research, and she loves supporting open science and learning about new research.
In our last post, we talked about the first base transversion editors: CGBEs, or C → G Base Editors. CGBEs first convert a cytosine (C) to uracil (U), just like Cytosine Base Editors (CBEs). But unlike CBEs, CGBEs then excise the U to create an abasic (empty) DNA site using ...
The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base editors (ABEs) chemically modify target bases without breaking the DNA backbone, making them efficient and precise tools for altering DNA sequences. These first base editors ...
We recently updated our blog post on Prime Editing, and that meant rereading many of the original papers reporting various prime editing tools. These papers are chock full of great tips to guide your experimental design, especially the design of the RNA sequences you’ll use in ...
A viral vector that can target specific tissues, even when administered systemically, without causing disease? Recombinant adeno-associated viral vectors, or rAAVs, can sound almost too good to be true! In a previous post, we covered systemic capsids, which allow AAVs to broadly ...
We’re big fans of all our depositors here at Addgene — your contributions make the repository what it is. So we’re thrilled this week to congratulate depositor David Baker, who was awarded the Nobel Prize in Chemistry last week alongside Demis Hassabis and John Jumper! The Baker ...
Western blots are a great tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a lot of work — and what if you want to detect more than one protein in your sample? Or what if something weird happened during your western and your ...
CRISPR is a sleek acronym for a real mouthful of a phrase: Clustered Regularly Interspaced Short Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too. CRISPR is an elegant bacterial immune system and an efficient gene editing tool… but ...
Have you ever designed a CRISPR guide RNA and wondered why it is limited to only 20 bases, or why it’s so important to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever more ubiquitous tool for genome engineering, and studying its ...
