Emily P. Bentley is a Blog Writer at Addgene. She earned her PhD in molecular biology and biophysics from Scripps Research, and she loves supporting open science and learning about new research.
Have you ever designed a CRISPR guide RNA and wondered why it is limited to only 20 bases, or why it’s so important to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever more ubiquitous tool for genome engineering, and studying its ...