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Alyssa Cecchetelli

Alyssa Cecchetelli is a Quality Control Scientist at Addgene. She received her PhD from Northeastern University studying cell signaling and communication in C. elegans. Alyssa is particularly interested in molecular biology, science communication, all NY sports teams and traveling. Follow her on twitter at @ACecchetelli

Blog articles by Alyssa Cecchetelli

Plasmids 101

Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

Alyssa Cecchetelli June 25, 2019

Horizontal gene transfer (HGT) is the movement of genetic material between organisms. It plays a key role in bacterial evolution and is the primary mechanism by which bacteria have gained antibiotic resistance and virulence. Scientists have studied how HGT occurs in nature and ...
transformation transduction conjugation transfection
Fluorescent Proteins

FlipGFP, a novel fluorescence protease reporter to study apoptosis

Alyssa Cecchetelli May 21, 2019

Apoptosis or “programmed cell death" plays a pivotal role in an array of biological processes including development, the immune system, and cell turnover. Apoptosis is a highly controlled process that is triggered by internal and external signals such as developmental cues and ...
FLipGFP
Addgene News

Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

Alyssa Cecchetelli April 2, 2019

Addgene recently moved to our brand new location, with more space to grow and help scientists share resources in new ways. Along with a new location comes the need for new conference room names. The Addgenie’s favorite conference room naming scheme? Gene names! This is not ...
Spaghetti Squash Addgene
Plasmids 101

Plasmids 101: Biotinylation

Alyssa Cecchetelli November 15, 2018

Biotin and its binding partner avidin are commonly used today in molecular biology for an array of different techniques and protocols. In this post we will discuss the natural role of biotin, biotinylation, the discovery of the biotin-avidin interaction and the uses of ...
Biotin and Avidin
CRISPR

New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

Alyssa Cecchetelli October 4, 2018

CRISPR pooled libraries have allowed scientists to easily perform genome-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to knock out, inhibit or activate target genes by combining specific sgRNAs with Cas9 or Cas9 derivatives.
Doench Libraries blog graphic
Plasmids 101

Plasmids 101: Protein Expression

Alyssa Cecchetelli June 7, 2018

The central dogma in molecular biology is DNA→RNA→Protein. To synthesize a particular protein DNA must first be transcribed into messenger RNA (mRNA). mRNA can then be translated at the ribosome into polypeptide chains that make up the primary structure of proteins. Most ...
Protein expression

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