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Alyssa Cecchetelli

Alyssa D. Cecchetelli is a Scientist at Addgene. She received her PhD from Northeastern University and is particularly interested in cell signaling and communication. She loves being able to help the scientific community share plasmids.

Recent Posts

Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

Posted by Alyssa Cecchetelli on Jul 7, 2020 9:15:00 AM

Base editors create specific point mutations in the genome, but they’re inefficient compared to CRISPR/Cas9 edits that rely on double strand DNA breaks. Due to this inefficiency it is crucial for scientists to not only easily identify base editing events in real-time but also enrich for base-edited cells in their experiments. In the past few years, scientists have created an array of base editing reporters that can help you do just that.

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Topics: CRISPR, Base Editing

Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

Posted by Alyssa Cecchetelli on May 26, 2020 9:15:00 AM

If you’re using CRISPR to make a genome edit, how do you know if your CRISPR experiment was successful in your organism or cell type? You can use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency of an experiment and confirm the correct edit was made to the genome without any off target effects. However, these methods can be labor intensive and quite time consuming.  

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Topics: CRISPR, Other CRISPR Tools

Finding nucleic acids with SHERLOCK and DETECTR

Posted by Alyssa Cecchetelli on Apr 16, 2020 9:00:09 AM

Originally published Aug 30, 2018 and updated April 16, 2020.

Sensitive and specific nucleic acid detection is crucial for clinical diagnostics, genotyping, and biotechnological advancements. Many methods of nucleic acid detection however, either lack the sensitivity or the specificity to detect nucleic acids at low concentrations and/or are too expensive, time-consuming, and complex to use outside of standard laboratories. In the case of the COVID-19 pandemic, qPCR can be used to diagnose the presence of SARS-CoV-2 RNA, but inadequate access to reagents and equipment has become a bottleneck.

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Topics: CRISPR, Cas Proteins, Other CRISPR Tools, COVID-19

Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Posted by Alyssa Cecchetelli on Dec 2, 2019 9:00:16 AM

There are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells (Milo, 2013). The number of interactions between these proteins is hard to imagine yet alone study.

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Topics: Plasmid Tags, Plasmids

HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

Posted by Alyssa Cecchetelli on Oct 10, 2019 8:41:58 AM

Part stable scaffold, part epitope binding region, all fused to a fluorescent protein. “Frankenbody,” like the infamous frankenstein, was constructed by grafting different parts together. By combining two optimized elements of an antibody probe, scientists can now more easily visualize single molecules and newly formed proteins. 

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Topics: Fluorescent Proteins, Localization with Fluorescent Proteins

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