By Chonnettia Jones
Read More
Ah, the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today we’ll cover the normalization and loading controls needed for relative quantification of a western blot — and why you might want to be careful relying on so called ...
In our last post, we talked about the first base transversion editors: CGBEs, or C → G Base Editors. CGBEs first convert a cytosine (C) to uracil (U), just like Cytosine Base Editors (CBEs). But unlike CBEs, CGBEs then excise the U to create an abasic (empty) DNA site using ...
The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base editors (ABEs) chemically modify target bases without breaking the DNA backbone, making them efficient and precise tools for altering DNA sequences. These first base editors ...
What’s in a type? That which we call CRISPR, by any other name would…probably still edit genomes.
Every few months, we highlight some of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.
Twenty years of accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs enabled by shared resources. As we step into 2025, these milestones remind us of the extraordinary impact that happens when scientists come together ...
Early CRISPR applications were often limited by the low editing efficiency of homology-directed repair (HDR), the pathway for resolving DNA double-strand breaks (DSBs) preferred by researchers. Compared to non-homologous end joining (NHEJ), HDR occurs at a relatively low ...
