By Susanna Bachle
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Adenoviral vectors (AdV) are attractive vectors for research applications and gene therapy: they can be produced at high titers, can accommodate large transgenes, transduce quiescent and dividing cells, and do not integrate into the host’s genome. The main challenge with using ...
Why study neural connectivity? One of the early lessons many of us learned in biology is that the body’s architecture and plumbing are important. We started with learning the head is connected to the neck. Shortly after, we learned about organs and the jobs they perform. This ...
Guest blogger Todd Waldman, Professor at Georgetown University, contributed to this post. Adeno-associated viruses (AAVs) make fantastic gene delivery vehicles for episomal gene expression and are particularly useful for gene delivery to the nervous system. For many years they ...
Many of us take comfort in the fact that it’s often not quantity, but quality that really matters. Well, it turns out this isn’t the case for using AAV. When it comes to infecting cells, titer, the amount of virus used, really does matter. (*psst*, quality definitely also ...
As the saying goes, “failing to plan is planning to fail.” Preparation is a key step to any experiment and can help prevent future headaches. To help you plan PEI transfections for AAV packaging, considering using this PEI Calculator. AAV packaging typically requires ...
Addgene recently attended the Society for Neuroscience 2017 conference in Washington DC (#SfN17). This massive conference attracted over 30,000 attendees (scientists and nonscientists alike). My only in-depth exposure to neuroscience prior to this conference was a rotation in ...
Remember the game show “The $25,000 Pyramid” where one player tries to get the other to guess a category by listing off things that fall into that category? Okay, let’s play. I’ll list the examples and you try to guess the category: ELISA... qPCR... Digital droplet PCR... DNA ...
