By Tyler Ford
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Many of us take comfort in the fact that it’s often not quantity, but quality that really matters. Well, it turns out this isn’t the case for using AAV. When it comes to infecting cells, titer, the amount of virus used, really does matter. (*psst*, quality definitely also ...
As the saying goes, “failing to plan is planning to fail.” Preparation is a key step to any experiment and can help prevent future headaches. To help you plan PEI transfections for AAV packaging, considering using this PEI Calculator. AAV packaging typically requires ...
Addgene recently attended the Society for Neuroscience 2017 conference in Washington DC (#SfN17). This massive conference attracted over 30,000 attendees (scientists and nonscientists alike). My only in-depth exposure to neuroscience prior to this conference was a rotation in ...
Remember the game show “The $25,000 Pyramid” where one player tries to get the other to guess a category by listing off things that fall into that category? Okay, let’s play. I’ll list the examples and you try to guess the category: ELISA... qPCR... Digital droplet PCR... DNA ...
This post was contributed by guest blogger Harshana S De Silva Feelixge. Gene therapy technologies hold great promise for improving or potentially curing human diseases that were previously thought to be incurable. Rapid advances in next generation sequencing technologies have ...
Adeno-associated viral (AAV) vectors are the most frequently used gene-transfer tools in the study of the brain and spinal cord, which together are known as the central nervous system (CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate, 2) they have ...
Updated Jun 1, 2021 by Meghan Rego. Reproducible data are key to science, so scientists are used to repeating experiments to confirm their findings. But no scientist wants to repeat an experiment because of poor reagent quality. To make sure our AAV vectors are of the highest ...
