Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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The RepCap plasmid consists of three promoters within the rep coding region that encodes four different Rep proteins. This region also encodes three different VP proteins, the MAAP protein, and the AAP protein.
Graphic of channelrhodopsin activated with blue light. Without light, the channelrhodopsin is inactive, no ions flow in, and neurons don't fire. With blue light, the channelrhodopsin opens and allows an influx of ions into the neuron. This results in neuronal firing.
An AAV with the viral genome inside. This zooms into the the AAV viral genome that is labeled. The viral genome is 4.4 kb maximum, and includes an ITR, promoter, and trangene. Option elements are LoxP sites, WPRE, and pA.

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