Plasmids 101: Modular Cloning

By Susanna Stroik

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Table outlining the features described in-text of each antibiotic
Cover page of the plasmids 101 eBook
Graphic showing the workflow for high complexity Golden Gate Assembly, starting with destination vector and PCR amplification of fragments, which combined in a single-tube reaction with enzyme and DNA ligase to produce the assembled DNA product.
Examples of gene, enhancer, and promoter traps at a genomic locus.
On the left is a pMD2.G plasmid, 5822 bp, depicted as a circle with a CMV enhancer marked and arrows showing different plasmid components. On the right is a plasmid, pMD2.G Dimer, 11,644 bp, depicted as a circle with two CMV enhancers, and doubled plasmid components compared to the first image, represented by arrows.
Repressible promoters Schematic-01

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