By Guest Blogger
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In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more in-depth, but we hope that this blog series will help you find new CRISPR tools for your research.
This post was contributed by guest blogger Pooran Dewari, a postdoc in Steve Pollard’s lab at the MRC Centre for Regenerative Medicine (CRM), Edinburgh. Most commercial antibodies do not work in pull-down assays: Epitope tagging provides a solution Proteins - the workhorses of ...
This article was contributed by Jessica Roginsky, Scientific Support Lead at Synthego. Article source: Step-by-Step Guide for Analyzing CRISPR Editing Results with ICE on Synthego’s blog. CRISPR-based genome engineering has revolutionized the gene editing field by making ...
CRISPR genome editing has made it easier to create knockout alleles in a variety of species, including the standard laboratory mouse. It’s also made targeted insertions relatively simple in C. elegans and bacteria. But CRISPRing typical mouse models, including creating ...
This post was contributed by guest blogger, Aneesh Karve, CTO at Quilt Data. This post was originally published on the Quilt Genomics Blog and is republished here with permission. Quilt is a collaborative database for genomics. In this article, Quilt CTO Aneesh Karve, shows how ...
Addgene is proud to announce that we recently acquired the ability to distribute plasmids with the piggyBac™ transposon. These plasmids, when combined with a source of piggyBac™ transposase (available from a licensed distributor) allow you to quickly transfer a DNA sequence from ...
This post was contributed by guest bloggers Albert Cheng and Mark Wanner. CRISPR-Cas9 offers a leap forward for genome editing, providing researchers with greatly enhanced accuracy, efficiency, and versatility. It has led to a tremendous acceleration of biomedical research, ...
