This post was contributed by guest blogger Pooran Dewari, a postdoc in Steve Pollard’s lab at the MRC Centre for Regenerative Medicine (CRM), Edinburgh. Most commercial antibodies do not work in pull-down assays: Epitope tagging provides a solution Proteins - the workhorses of ...
This article was contributed by Jessica Roginsky, Scientific Support Lead at Synthego. Article source: Step-by-Step Guide for Analyzing CRISPR Editing Results with ICE on Synthego’s blog. CRISPR-based genome engineering has revolutionized the gene editing field by making ...
CRISPR genome editing has made it easier to create knockout alleles in a variety of species, including the standard laboratory mouse. It’s also made targeted insertions relatively simple in C. elegans and bacteria. But CRISPRing typical mouse models, including creating ...
This post was contributed by guest blogger, Aneesh Karve, CTO at Quilt Data. This post was originally published on the Quilt Genomics Blog and is republished here with permission. Quilt is a collaborative database for genomics. In this article, Quilt CTO Aneesh Karve, shows how ...
Addgene is proud to announce that we recently acquired the ability to distribute plasmids with the piggyBac™ transposon. These plasmids, when combined with a source of piggyBac™ transposase (available from a licensed distributor) allow you to quickly transfer a DNA sequence from ...
If you follow CRISPR research, you know all about using non-homologous end-joining (NHEJ) to make deletions or homology-directed repair (HDR) to create precise genome edits. But have you heard of another double-stranded break repair mechanism: MMEJ (microhomology-mediated ...
This post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software and the piñata effect Two years ago I was a part of a group (Biology of Host-parasite Interactions, Institut Pasteur, Paris) that changed genome editing in the malaria community ...
As Eric Mendenhall of the University of Alabama in Huntsville explains it, a major goal in his laboratory is to understand the function of the non-coding portion of the genome. Mendenhall and Richard Myers of HudsonAlpha (where Mendenhall is also an adjunct faculty member) have ...
