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This post was contributed by guest blogger Erik L. Snapp. Stop using EGFP/GFP for fusion proteins! Despite multiple studies in high profile journal articles, many researchers remain unaware that EGFP/GFP is prone to forming noncovalent dimers. This property of EGFP can lead to ...
At Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse protein even more useful! From FPs optimized for oxidizing environments to photoconvertible variants, it seems like GFP is always learning new things. Now, work from Connie ...
Epigenetics has recently been hitting the headlines, with sotires like the potential devastation of the palm oil industry through epigenetic effects on the Cover of Nature. So what is epigenetics and what tools are available to study it?
This post was contributed by guest bloggers Erik L. Snapp and Lindsey M. Costantini. "You underestimate the power of the Dark Side." --Darth Vader in "Return of the Jedi" While Vader was referring to the evil side of a mystical "Force," this quote is equally applicable to many ...
Since the first research applications of GFP were published in the 1990s, biologists have spent a lot of time making things glow. Chances are you’ve used a GFP derivative to conduct subcellular localization studies or make a reporter construct. Fluorescent proteins (FPs) are ...
This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery at the University of Miami. The beginning of this century has seen some major advances in light microscopy, particularly related to the neurosciences. These developments in ...
CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation systems remain valuable to biology. Cre-lox recombination, discovered in the 1980s, is one of the most important ways to spatially and temporally control gene expression, ...
