By Benoit Giquel
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This post was contributed by Kurt Thorn of the Nikon Imaging Center at UCSF. A common requirement for live cell imaging experiments is the ability to follow multiple fluorescently tagged species simultaneously. To do so with fluorescent protein labels requires multiple ...
Updated May 10, 2021. In the world of fluorescent proteins and their use for imaging cell biology, Michael Davidson’s lab at Florida State University has been the go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive 1,350 scientists from more than ...
Luciferases are a class of enzymes capable of catalyzing chemical reactions in living organisms resulting in the emission of photons. The most familiar bioluminescent organism for most people is the firefly (Photinus pyralis) and perhaps not surprisingly it is also the most ...
This post was contributed by Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how fluorescent proteins glow? Fluorescent Proteins (FPs) were first discovered over 50 years ago, with the discovery of the Green Fluorescent Protein (GFP), a protein from the ...
Bioluminescence and fluorescence from proteins such as Green Fluorescent Protein (GFP) has likely existed in creatures such as jellyfish for millions of years; however, it took until the 1960s for scientists to begin to study GFP and deduce its biochemical properties. Now GFP ...
Oliver Griesbeck of the Max Planck Institute for Neurobiology has been working on genetically encoded indicators of calcium and other small molecules since the very beginnings of the field. Those engineered sensors were designed to replace synthetic calcium dyes, which had been ...
If you start poking around on Addgene’s Fluorescent Protein Guide to In Vivo Imaging, you’ll pretty quickly notice the name Vladislav Verkhusha popping up again and again, and for good reason. We all know scientists have used fluorescent proteins to observe what’s happening ...
