By Mary Gearing
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This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery at the University of Miami. The beginning of this century has seen some major advances in light microscopy, particularly related to the neurosciences. These developments in ...
CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation systems remain valuable to biology. Cre-lox recombination, discovered in the 1980s, is one of the most important ways to spatially and temporally control gene expression, ...
The first time I heard about FRET during a journal club, my guitarist brain automatically thought about the raised element found on the neck of my guitar...not really useful for a biologist you would say. The student was of course talking about the now well-known FRET, aka ...
This post was contributed by Kurt Thorn of the Nikon Imaging Center at UCSF. A common requirement for live cell imaging experiments is the ability to follow multiple fluorescently tagged species simultaneously. To do so with fluorescent protein labels requires multiple ...
Updated May 10, 2021. In the world of fluorescent proteins and their use for imaging cell biology, Michael Davidson’s lab at Florida State University has been the go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive 1,350 scientists from more than ...
This post was contributed by Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how fluorescent proteins glow? Fluorescent Proteins (FPs) were first discovered over 50 years ago, with the discovery of the Green Fluorescent Protein (GFP), a protein from the ...
Bioluminescence and fluorescence from proteins such as Green Fluorescent Protein (GFP) has likely existed in creatures such as jellyfish for millions of years; however, it took until the 1960s for scientists to begin to study GFP and deduce its biochemical properties. Now GFP ...
