Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger, Addgene Advisory Board member, and Institute Scientist at the Broad Institute, John Doench. CRISPR technology has made it easier than ever both to engineer specific DNA ...
Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel. CRISPR genome editing has quickly become a popular system for in vitro and germline genome editing, but in vivo gene editing approaches have been limited by problems with Cas9 delivery. ...
Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy to target multiple loci - a concept called multiplexing. Since CRISPR is such a robust system, editing or labeling efficiency doesn’t usually change when you add multiple ...
This blog post was originally written by Caroline LaManna, published Mar 8, 2016. The updated and expanded version by Nyla Naim was published Sept 3, 2020. Scientists around the world have been making major improvements to CRISPR technology since its initial applications for ...
Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired genomic target provided that (1) the sequence is unique compared to the rest of the genome and (2) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM ...
Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh et al., 2016) as a RNA-targeting CRISPR enzyme, they adapted Cas13b for ...
Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used for genome editing, activating gene expression, repressing gene expression, and much more. But there’s one thing ...
Base editors create specific point mutations in the genome, but they’re inefficient compared to CRISPR/Cas9 edits that rely on double strand DNA breaks. Due to this inefficiency it is crucial for scientists to not only easily identify base editing events in real-time but also ...
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