By Eddy Page
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My lab's vector of choice is AAV, with nearly every experiment requiring AAV. Before joining my lab, I had never worked with AAV, so naturally I had to package some virus for my first experiment. It was a bit intimidating, but I had my lab’s protocols and some great co-workers ...
Background on neuronal tracing A key aspect to understanding the brain’s function is knowing its architecture, in particular the connections between different brain regions. For example, communication between the hippocampus and the prefrontal cortex brain regions is involved in ...
It was by serendipity that I got into the field of gene therapy, more specifically AAV-based retinal gene therapy. The year was 2001 and I started a job as a technician in a lab using adeno-associated viral vectors (AAVs) to treat an inherited retinal degenerative disease called ...
Guest blogger Todd Waldman, Professor at Georgetown University, contributed to this post. Adeno-associated viruses (AAVs) make fantastic gene delivery vehicles for episomal gene expression and are particularly useful for gene delivery to the nervous system. For many years they ...
Many of us take comfort in the fact that it’s often not quantity, but quality that really matters. Well, it turns out this isn’t the case for using AAV. When it comes to infecting cells, titer, the amount of virus used, really does matter. (*psst*, quality definitely also ...
Addgene recently attended the Society for Neuroscience 2017 conference in Washington DC (#SfN17). This massive conference attracted over 30,000 attendees (scientists and nonscientists alike). My only in-depth exposure to neuroscience prior to this conference was a rotation in ...
Remember the game show “The $25,000 Pyramid” where one player tries to get the other to guess a category by listing off things that fall into that category? Okay, let’s play. I’ll list the examples and you try to guess the category: ELISA... qPCR... Digital droplet PCR... DNA ...
