Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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This post was contributed by guest blogger Meghan Monroy, a graduate student in Protein Science at the University of Connecticut. Molecular cloning is the isolation, insertion and amplification of a recombinant DNA without sequence alteration. Molecular cloning techniques are ...
Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The technique relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to ...
This post was contributed by Future of Research Executive Director, Gary McDowell. On Dec 1st, the threshold at which salaried workers receive overtime payment for working more than 40 hours per week will increase from $23,660 to $47,476 per year, under updates to the Fair Labor ...
Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything”) on r/Science. You can see some of Joanne’s comments on the AMA process below, but we also wanted to share some thoughts on why we decided to do an AMA in the first ...
This post was contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No matter what facet of academia you are in, grant writing can be the lynch pin that results in your success or failure and demands attention, practice, and honing of your skills ...
This post was contributed by guest blogger Gairik Sachdeva. Healthcare consulting is a fast-paced field, requiring people who are willing to quickly learn, and apply their knowledge to a variety of problems. In this post, I’ll share what I’ve learned as a healthcare consultant ...
Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the genome engineering world by storm owing to its ease of use and utility in a wide variety of organisms. While much of current CRISPR research focuses on its potential ...
