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Visualizing Protein Turnover In Situ

Posted by Guest Blogger on Jan 16, 2018 10:20:10 AM

This post was contributed by guest blogger, Eugenia Rojas.

A question worthy of a PhD: How do you visualize protein turnover within a neuron?

For my PhD I studied a synaptic protein that is linked to neurodegeneration. The level of this protein is decreased in Alzheimer’s disease patient’s brains. However, it is not known why or how this happens. Therefore, I set out to study how protein turnover is regulated in neurons.

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Topics: Blog

Transferable Skills Guide: Identifying Your Transferable Skills

Posted by Kayla Strickland on Jan 11, 2018 10:29:18 AM

What is a transferable skill?

Time management. I needed it when balancing a handful of demanding courses, a capstone paper I really wanted to hit out of the park, part time work, bills, (at times) a social life and rest. I need it just as much in my current role as Customer Support and Operations Manager at Addgene. In this role, I balance my daily tasks, meet cross-team project commitments, respond to any issues raised by team members, and plan for the future of the team. All while still paying bills and having a life outside my job.

The same can be said about teamwork, communication, writing, management, and creativity; I have developed these skills through school, jobs, and volunteer work, and I guarantee you have developed them through similar experiences in graduate school. These skills will be useful anywhere I work in the future; they are transferrable across most, if not all, industries and work environments. This is why they are called transferable skills.

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Topics: Career, Transferable Skills Guide

Top Requested Plasmid of 2017 - pMD2.G

Posted by Tyler Ford on Jan 10, 2018 11:13:59 AM

Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances biological research and discovery. CRISPR is a great example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9 and other genes to mammalian cells. For this reason, the top requested plasmid of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab!

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Topics: Plasmid Technology, Hot Plasmids, Viral Vectors

15 Hot Plasmids from 2017

Posted by Tyler Ford on Jan 9, 2018 10:02:27 AM

Every quarter we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles provide a synopsis of a plasmid or group of plasmids' functions and applications. We hope that these articles make it easier for you to find and use the plasmids you need. You can find all the hot plasmids from 2017 below. With over 50,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but be sure to let us know if you'd like to write about your plasmids in a future blog post. No time to read?

Listen to our hot plasmids segment on the Addgene Podcast.

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Topics: Hot Plasmids, News

Identifying Sequence Elements with SnapGene's Feature Database

Posted by Guest Blogger on Dec 21, 2017 9:06:58 AM

This post was contribued by guest bloggers Aline and Benjamin Glick from SnapGene.

SnapGene was created to meet a need. While there were software tools available to biomedical researchers manipulating DNA sequences on a daily basis, many found these tools inadequate for planning, visualizing, and documenting their procedures. Preventable errors in the design of cloning strategies set experiments back days or even weeks. Primer design was done painstakingly by hand. Records of plasmid construction were often incomplete or nonexistent. In the 21st century, many molecular biologists didn’t know the complete sequences or properties of the DNA molecules they were using.

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Topics: Plasmid Technology, Blog

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