Hot Plasmids and Viral Preps - January 2021

By Various Addgenies

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Addgene presents... the 12 days of CRISPR #12DaysOfCRISPR
A schematic of the cell membrane with a GPCR and the transducer complex. When the GPCR binds the ligand, the transducer complex dissociates resulting in a decrease in BRET.
Multiplexed insertion is possible using a single plasmid that contains a multi-spacer CRISPR array. This encodes separate gRNAs that target different locations in the genome.
The RepCap plasmid consists of three promoters within the rep coding region that encodes four different Rep proteins. This region also encodes three different VP proteins, the MAAP protein, and the AAP protein.

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