Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Previously, we provided a general overview of the QC process at Addgene. All plasmids go through this same initial QC process using an Illumina MiSeq, but that’s not the only technology Addgene uses to ensure accuracy of deposited plasmids. To resolve QC issues, assembly issues, ...
This post was written by Alfonso Timoneda, with significant contributions from Robert Hurt and Mohamed Soufi. So you’ve learned about the Modular Cloning (MoClo) system and what it can do for you, and you’ve chosen one of the MoClo kits available through Addgene that suits your ...
You have just purified your plasmid and are ready to move on to your downstream application — but wait! Do you know how much plasmid DNA you have or how pure your sample really is?
We all know the timeline for publishing research papers can be long and unpredictable. Fortunately, you can submit plasmids at any point in that process and we provide an option to keep your deposit on hold from public access until the article is published. This policy gives us ...
An oldie but a goodie — a phrase used to refer to something that has fallen out of fashion but is still useful and most importantly, effective. In the research world, there is a myriad of tools this could be applied to. When it comes to altering gene expression, CRISPR ...
These days, it hardly seems like we finish writing about one dual CRISPR-transposon system before another exciting new advance emerges! The programmability and targeting power of CRISPR combined with the large sequence capacity of transposons open whole new worlds to explore. ...
Every few months we highlight some of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.
