The Addgene Blog

Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.

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Recent Posts

Cartoon of Addgene's mascot Blugene
Comparison of shRNA and miRNA processing pathways.
Cartoon of CRISPR-associated transposase (CAST) integrating donor DNA into a genomic target site.
A cartoon plasmid at the center of the image has arrows leading to many possible host species: gram negative bacteria, gram positive bacteria, yeast, and mammalian cells.
A cartoon depiction of a lentiviral genome forming an episome in the presence of non-functional LV integrase. The linear lentiviral genome has a long terminal repeat (LTR) on each end. A split arrow shows two paths for circular episome formation. Homologous recombination within the LTRs results in a single LTR in the circular episome. Non-homologous end joining results in two adjacent LTRs in the circular episome.

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