Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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While monoclonal and polyclonal antibodies are readily available from several sources, fewer sources of recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and rAb plasmids!). Since recombinant antibodies conveniently allow for ...
I never would have imagined that my summer before high school senior year would have been spent within a lovable community that has allowed me to expand my knowledge more than ever before. Before joining Addgene, I had spent most of my junior year stressed out applying for ...
The widespread use of γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted the development of multiple virus packaging methods. Across these methods, the following components are needed:
Western blots are a great tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a lot of work — and what if you want to detect more than one protein in your sample? Or what if something weird happened during your western and your ...
As part of Addgene’s 20th anniversary celebration, we’ve been finding and sharing stories unique to our repository and resources. As editor of Addgene’s eBooks — including the recently released Viral Vectors 101 2nd edition — I was incredibly excited when we picked our viral ...
An enzyme-linked immunosorbent assay (ELISA) is a versatile method used to quantify the level of target antigen in a sample. While Engvall et al. originally developed the ELISA assay to measure antibody levels, scientists have since adapted it for a host of different proteins ...
If you’ve deposited or requested plasmids from Addgene, then you’ve been involved in the material transfer agreement (MTA) process. Even though this process is essential to sharing plasmids around the globe, it can be a bit of a mystery for those who aren’t familiar with it. If ...
