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In flow cytometry, compensation is the process of correcting spillover from one fluorescent channel to another. When you label your samples with multiple antibodies, the fluorescent probes on the antibodies may have similar emission spectra, meaning they will emit fluorescent ...
Modular cloning is a popular DNA assembly tool used to build single- and multi-gene constructs for a variety of applications. MoClo tools can be used in bacterial and cell-free expression systems, mammalian cells, yeast, flies, and even plants! In this blog we will review common ...
The UN General Assembly has declared 2024 the International Year of Camelids. The declaration is intended to raise awareness of the economic and cultural importance of these animals to human populations around the world. Here at Addgene, we love camelids too, though not just for ...
FITS In vitro CRISPR screens in immune cells have identified many putative regulators of immunity. However, in vitro screens cannot faithfully model the complex spatial, cellular, metabolic, and cytokine microenvironment that exists in vivo. Over the past five years, in vivo ...
The triple T’s of biology are transformations, transfections, and transductions! In this blog we will discuss all things transductions. If you’ve ever wondered how they are different from or similar to the other Ts, we have your answers. If you know the basics but are here ...
In the pursuit of knowledge and groundbreaking discoveries, the world of academia often emphasizes structured methodologies and rigorous research plans. However, amidst the organized chaos of lab research and PhD studies, there lies a hidden gem that has the potential to ...
As we celebrate twenty years at Addgene, we are sharing stories of our repository’s positive impact on science and scientists around the globe. Our first story focuses on a technology that has changed virtually all of biology…CRISPR.
